LTA anticorps (AA 49-194)

N° du produit ABIN1860795

Détail du produit anti-LTA anticorps

Antigène: LTA (Lymphotoxin-alpha (LTA))

Épitope: AA 49-194

Reactivité: Lapin

Hôte: Cobaye

Clonalité: Polyclonal

Conjugué: Cet anticorp LTA est non-conjugé

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Séquence: MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- GH STLKPAAHLV GDPSAQDSLR WRANTDRAFL RHGFSLSNNS LLVPSSGLYF VYSQVVFSGE GCSPKAVPTP LYLAHEVQLF SSQYSFHVPL LSAQKSVCPG PQGPWVRSVY QGAVFLLTQG DQLSTHTDGI AHLLLSPSSV FFGA

Specificité: It has been selected for its ability to recognize TNFb in immunohistochemical staining and Western blotting.

Purification: Affinity Chromatography

Immunogène: TNFb (AA 49-194)

Isotype: IgG

Information d'application

Indications d'application: Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.

Commentaires:

Content: The quality control contains recombinant TNFb (Gly49~Ala194) disposed in loading buffer.
Usage: 10 µL per well when 3,3'-Diaminobenzidine(DAB) as the substrate. 5 µL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
Loading Buffer: 100 mM Tris(pH8.8), 2 % SDS, 200 mM NaCl, 50 % glycerol,BPB 0.01 % , NaN3 0.02 % .

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02 % NaN3, 50 % glycerol.

Agent conservateur: Sodium azide

Précaution d'utilisation: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Conseil sur la manipulation: Avoid repeated freeze/thaw cycles

Stock: 4 °C

Stockage commentaire: Store at 2-8 °C for one month. Aliquot and store at -80 °C for 12 months.

Date de péremption: 12 months

Détails sur LTA

Antigène: LTA (Lymphotoxin-alpha (LTA))

Autre désignation: Tumor Necrosis Factor Beta (TNFb) (LTA Produits)

Synonymes: anticorps LT, anticorps TNFB, anticorps TNFSF1, anticorps LT-[a], anticorps LT-alpha, anticorps LT[a], anticorps LTalpha, anticorps Ltx, anticorps TNF-beta, anticorps Tnfb, anticorps Tnfsf1b, anticorps hlb382, anticorps lymphotoxin alpha, anticorps lymphotoxin A, anticorps LTA, anticorps Lta

Classe de substances: Chemical

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process