CHEK1 anticorps (AA 1-474)

N° du produit ABIN187834

Cet anticorps Mouton Polyclonal détecte spécifiquement CHEK1 dans WB. Il présente une réactivité avec des échantillons de Humain, Souris, Rat et Singe.

Aperçu rapide pour CHEK1 anticorps (AA 1-474) (ABIN187834)

Antigène: CHEK1 (Checkpoint Kinase 1 (CHEK1))

Reactivité: Humain, Souris, Rat, Singe

Hôte: Mouton

Clonalité: Polyclonal

Conjugué: Cet anticorp CHEK1 est non-conjugé

Application: Western Blotting (WB)

Détail du produit anti-CHEK1 anticorps

Épitope: AA 1-474

Specificité: Recognizes chk1 kinase. Species Reactivity: Human, monkey, mouse, and rat, other species not tested. Molecular Weight: .54 kDa. Cellular Location: Cytoplasmic.

Stérilité: 0.2 μm filtered

Immunogène: GST fusion protein corresponding to amino acid sequence 1-474 of human chk1 kinase.

Information d'application

Indications d'application: Western blot: Use 1-10 µg/ml. The optimal dilution for a specific application should be determined by the researcher.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Provided as 0.2 µm sterile filtered solution in PBS with 0.08% sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Détails sur CHEK1

Antigène: CHEK1 (Checkpoint Kinase 1 (CHEK1))

Autre désignation: CHK1

Sujet: One mechanism by which checkpoints maintain the fidelity of cell cycle events is through blocking mitosis in response to unreplicated or damaged DNA. In most species this occurs through inhibiting activation of the cyclic-dependent kinase cdc2, which regulates entry into mitosis. Chk1, a kinase involved in the DNA damage checkpoint response can phosphorylate Cdc25C an activator of cdc2. It is hypothesized that chk1 induces serine 216 phosphorylation of Cdc 25C and subsequent binding negatively regulated Cdc25C, thus preventing it from activating cdc2.

Pathways: Signalisation p53, Apoptose, Cycle Cellulaire, Réparation de l'ADN