HLA-DQB1 anticorps (N-Term)

N° du produit ABIN1881422

Détail du produit anti-HLA-DQB1 anticorps

Antigène: HLA-DQB1 (Major Histocompatibility Complex, Class II, DQ beta 1 (HLA-DQB1))

Épitope: AA 13-39, N-Term

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp HLA-DQB1 est non-conjugé

Application: Western Blotting (WB)

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogène: This HLA-DQB1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 13-39 amino acids from the N-terminal region of human HLA-DQB1.

Isotype: Ig Fraction

Information d'application

Indications d'application: WB: 1:1000

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Date de péremption: 6 months

Références pour anticorps anti-HLA-DQB1 (ABIN1881422)

Larhammar, Hyldig-Nielsen, Servenius, Andersson, Rask, Peterson: "Exon-intron organization and complete nucleotide sequence of a human major histocompatibility antigen DC beta gene." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 80, Issue 23, pp. 7313-7, (1984) (PubMed).

Boss, Strominger: "Cloning and sequence analysis of the human major histocompatibility complex gene DC-3 beta." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 81, Issue 16, pp. 5199-203, (1984) (PubMed).

Larhammar, Andersson, Andersson, Bill, Böhme, Claesson, Denaro, Emmoth, Gustafsson, Hammarling: "Molecular analysis of human class II transplantation antigens and their genes." dans: Human immunology, Vol. 8, Issue 1, pp. 95-103, (1983) (PubMed).

Larhammar, Schenning, Gustafsson, Wiman, Claesson, Rask, Peterson: "Complete amino acid sequence of an HLA-DR antigen-like beta chain as predicted from the nucleotide sequence: similarities with immunoglobulins and HLA-A, -B, and -C antigens." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 79, Issue 12, pp. 3687-91, (1982) (PubMed).

Détails sur HLA-DQB1

Antigène: HLA-DQB1 (Major Histocompatibility Complex, Class II, DQ beta 1 (HLA-DQB1))

Autre désignation: HLA-DQB1 (HLA-DQB1 Produits)

Synonymes: anticorps CELIAC1, anticorps HLA-DQB, anticorps IDDM1, anticorps mhc-DRB, anticorps DLA-DQB, anticorps MAMU-DQB1, anticorps LOC100172120, anticorps DKFZp469C0237, anticorps major histocompatibility complex, class II, DQ beta 1, anticorps HLA-DQB1, anticorps PATR-DQB1, anticorps DLA-DQB1, anticorps MAMU-DQB1, anticorps LOC100172120

Sujet: Binds peptides derived from antigens that access the endocytic route of antigen presenting cells (APC) and presents them on the cell surface for recognition by the CD4 T-cells. The peptide binding cleft accommodates peptides of 10-30 residues. The peptides presented by MHC class II molecules are generated mostly by degradation of proteins that access the endocytic route, where they are processed by lysosomal proteases and other hydrolases. Exogenous antigens that have been endocytosed by the APC are thus readily available for presentation via MHC II molecules, and for this reason this antigen presentation pathway is usually referred to as exogenous. As membrane proteins on their way to degradation in lysosomes as part of their normal turn-over are also contained in the endosomal/lysosomal compartments, exogenous antigens must compete with those derived from endogenous components. Autophagy is also a source of endogenous peptides, autophagosomes constitutively fuse with MHC class II loading compartments. In addition to APCs, other cells of the gastrointestinal tract, such as epithelial cells, express MHC class II molecules and CD74 and act as APCs, which is an unusual trait of the GI tract. To produce a MHC class II molecule that presents an antigen, three MHC class II molecules (heterodimers of an alpha and a beta chain) associate with a CD74 trimer in the ER to form an heterononamer. Soon after the entry of this complex into the endosomal/lysosomal system where antigen processing occurs, CD74 undergoes a sequential degradation by various proteases, including CTSS and CTSL, leaving a small fragment termed CLIP (class-II-associated invariant chain peptide). The removal of CLIP is facilitated by HLA-DM via direct binding to the alpha-beta-CLIP complex so that CLIP is released. HLA-DM stabilizes MHC class II molecules until primary high affinity antigenic peptides are bound. The MHC II molecule bound to a peptide is then transported to the cell membrane surface. In B-cells, the interaction between HLA-DM and MHC class II molecules is regulated by HLA-DO. Primary dendritic cells (DCs) also to express HLA-DO. Lysosomal miroenvironment has been implicated in the regulation of antigen loading into MHC II molecules, increased acidification produces increased proteolysis and efficient peptide loading.

Poids moléculaire: 29991

NCBI Accession: NP_001230891, NP_002114

UniProt: P01920

Pathways: TCR Signaling, Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints