p53 anticorps (pSer315)
Aperçu rapide pour p53 anticorps (pSer315) (ABIN196788)
Antigène
Voir toutes p53 (TP53) AnticorpsReactivité
Hôte
Clonalité
Conjugué
Application
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Épitope
- pSer315
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Specificité
- This antibody detects endogenous levels of p53 only when phosphorylated at Serine 315.
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Purification
- Affinity Chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
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Immunogène
- The antiserum was produced against synthesized phosphopeptide derived from human p53 around the phosphorylation site of Serine 315 (S-S-SP-P-Q).
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Indications d'application
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Western Blot: 1/500-1/1000. Immunofluorescence: 1/100-1/200. Immunohistochemistry on Paraffin Sections: 1/50approx. 1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
Restrictions
- For Research Use only
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Concentration
- 1.0 mg/mL
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Buffer
- PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol.
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Agent conservateur
- Sodium azide
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Précaution d'utilisation
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Conseil sur la manipulation
- Avoid repeated freezing and thawing.
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Stock
- -20 °C
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Stockage commentaire
- Store the antibody (in aliquots) at -20 °C.
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- p53 (TP53) (Tumor Protein P53 (TP53))
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Autre désignation
- p53 (TP53)
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Sujet
- Synonyms: Cellular tumor antigen p53, NY-CO-13, Phosphoprotein p53, Tumor suppressor p53
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ID gène
- 7157
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NCBI Accession
- NP_000537
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UniProt
- P04637
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Pathways
- Signalisation p53, Signalisation MAPK, Signalisation PI3K-Akt, Apoptose, AMPK Signaling, Chromatin Binding, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Autophagy, L'effet Warburg
Antigène
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