Western Blot: 1: 500approx. 1: 1000. Immunohistochemistry: 1: 50approx. 1: 100. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Concentration
1.0 mg/mL
Buffer
PBS (without Mg2+ and Ca2+), pH 7.4 containing 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Conseil sur la manipulation
Avoid repeated freezing and thawing.
Stock
-20 °C
Antigène
HSP90AB1
(Heat Shock Protein 90kDa alpha (Cytosolic), Class B Member 1 (HSP90AB1))
anticorps D6S182, anticorps HSP84, anticorps HSP90B, anticorps HSPC2, anticorps HSPCB, anticorps GRP94, anticorps TRA1, anticorps hsp90b, anticorps 90kDa, anticorps AL022974, anticorps C81438, anticorps Hsp84, anticorps Hsp84-1, anticorps Hsp90, anticorps Hspcb, anticorps Hsp70, anticorps Hsp70-1, anticorps Hsp70.1, anticorps hsp68, anticorps HSP90-BETA, anticorps hsp90beta, anticorps wu:fa29f01, anticorps wu:fa91e11, anticorps wu:fd59e11, anticorps wu:gcd22h07, anticorps HSP90, anticorps heat shock protein 90 alpha family class B member 1, anticorps heat shock protein 90 beta family member 1, anticorps Heat Shock Protein 90, endoplasmic reticulum, anticorps heat shock protein 90B, anticorps heat shock protein 90 alpha (cytosolic), class B member 1, anticorps heat shock protein 1B, anticorps heat shock protein 90kDa alpha family class B member 1 S homeolog, anticorps heat shock protein 90, alpha (cytosolic), class B member 1, anticorps HSP90AB1, anticorps HSP90B1, anticorps HSP90B, anticorps hsp90ab1, anticorps Hsp90ab1, anticorps Hspa1b, anticorps hsp90ab1.S
Sujet
In mammalian cells there are at least two Hsp90 isoforms, Hsp90a and Hsp90b which are encoded by separate genes. These ubiquitous and highly conserved proteins account for 1-2 % of all cellular proteins in most cells. Hsp90 is part of the cells powerful network of chaperones to fight the deleterious consequences of protein unfolding caused by nonphysiological conditions. However, in the absence of stress, Hsp90 is a necessary component of fundamental cellular processes such as hormone signaling and cell cycle control. In this context several key regulatory proteins such as steriod receptors, cell cycle kinases involved in signal transduction and p53 have been identified as substrates of Hsp90. It has been suggested that Hsp90 acts as a capacitor for morphological evolution by buffering widespread variation, which may affect morphogenic pathways.Synonyms: HSP84, HSP90B, HSPC2, HSPCB, Heat shock 84 kDa, Heat shock protein HSP 90-beta