ERK1 anticorps (C-Term)

N° du produit ABIN199417

Cet anticorps Lapin Polyclonal détecte spécifiquement ERK1 dans WB, IHC, ELISA et IP. Il présente une réactivité avec des échantillons de Humain, Souris et Rat.

Aperçu rapide pour ERK1 anticorps (C-Term) (ABIN199417)

Antigène: ERK1 (MAPK3) (Mitogen-Activated Protein Kinase 3 (MAPK3))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp ERK1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP)

Détail du produit anti-ERK1 anticorps

Épitope: C-Term

Specificité: Reacts strongly with ERK1 and ERK2. A synthetic peptide, corresponding to a 15 amino acid sequence near the carboxyl terminus (339-353 aa of rat ERK1) was conjugated to carrier protein and used as the immunogen. Reactivity to ERK1 and ERK2 has been confirmed by western blot analysis of human A431 and K562 cells, mouse brain and rat brain. Human, mouse and rat (positive control: human A431 and K562 cells, mouse brain and rat brain cell lysates).

Purification: Immunoaffinity purified

Immunogène: Synthetic peptide (a.a. 339-353) corresponding to a C-terminal segment of rat ERK1.

Type of Immunogen: Synthetic peptide

Isotype: IgG

Information d'application

Indications d'application: Approved: ELISA (0.1 - 1 μg/mL), IHC (5 - 10 μg/mL), IP, WB

Usage: Suitable for use in ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry. ELISA: 0.1-1 μg/mL (to coat: 10 μg/mL). Immunoprecipitation: 2-5 μg. Immunohistochemistry: 5-10 μg/mL. Western blotting: ~1:1000. Positive control: Human A431 and K562 cells, mouse brain and rat brain cell lysates).

Commentaires:

Target Species of Antibody: Rat

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.05 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.

Détails sur ERK1

Antigène: ERK1 (MAPK3) (Mitogen-Activated Protein Kinase 3 (MAPK3))

Autre désignation: MAPK3 / ERK1

Sujet: Name/Gene ID: MAPK3
Subfamily: MAPK
Family: Protein Kinase

Synonyms: MAPK3, ERK-1, ERT2, HUMKER1A, HS44KDAP, MAP kinase 1, MAP kinase isoform p44, MAPK 1, MAPK 3, p44, p44MAPK, p44-MAPK, p44ERK1, Insulin-stimulated MAP2 kinase, MAP kinase 3, p44-ERK1, ERK1, PRKM3

ID gène: 5595

UniProt: P27361

Pathways: Signalisation MAPK, Signalisation RTK, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Response to Growth Hormone Stimulus, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Protein targeting to Nucleus, Toll-Like Receptors Cascades, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, VEGFR1 Specific Signals, S100 Proteins