ERK1 anticorps (pThr202, Tyr204)

N° du produit ABIN220153

Cet anticorps Lapin Polyclonal détecte spécifiquement ERK1 dans WB, IHC et IHC (p). Il présente une réactivité avec des échantillons de Humain et Rat.

Aperçu rapide pour ERK1 anticorps (pThr202, Tyr204) (ABIN220153)

Antigène: ERK1 (MAPK3) (Mitogen-Activated Protein Kinase 3 (MAPK3))

Reactivité: Humain, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp ERK1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-ERK1 anticorps

Épitope: pThr202, Tyr204

Specificité: Specific for ERK 1+2 when phosphorylated at Thr202 and Tyr204. On Western blots, it identifies two bands at ~42 and 44kD. Reactivity has been confirmed with UV-treated human Jurkat cell lysates. Immunolabeling in UV-treated Jurkat cells is blocked by the phosphopeptide, but not by the dephosphopeptide.

Purification: Purified

Immunogène: Synthetic phosphopeptide corresponding to the regions surrounding the phosphorylated Thr202 and Tyr204 residues of ERK1+2 (p44/42 MAP kinase).

Type of Immunogen: Synthetic phospho-peptide

Isotype: IgG

Information d'application

Indications d'application: Approved: IHC, IHC-P, WB (1:1000)

Usage: Suitable for use in Western Blot and Immunohistochemistry. Western Blot (rat): 1:1000. Immunohistochemistry, human (formalin fixed, paraffin embedded): 1:400. Staining of formalin-fixed, paraffin-embedded tissues, heat induced epitope retrieval with citrate buffer,  pH 6.0, is required prior to staining.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: 10 mM HEPES solution,  pH 7.5, 0.1 mg/mL BSA, 50 % glycerol.

Stock: 4 °C,-20 °C

Stockage commentaire: Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.

Détails sur ERK1

Antigène: ERK1 (MAPK3) (Mitogen-Activated Protein Kinase 3 (MAPK3))

Autre désignation: MAPK3 / ERK1

Sujet: Name/Gene ID: MAPK3
Subfamily: MAPK
Family: Protein Kinase

Synonyms: MAPK3, ERK-1, ERT2, HUMKER1A, HS44KDAP, MAP kinase 1, MAP kinase isoform p44, MAPK 1, MAPK 3, p44, p44MAPK, p44-MAPK, p44ERK1, Insulin-stimulated MAP2 kinase, MAP kinase 3, p44-ERK1, ERK1, PRKM3

ID gène: 5595

UniProt: P27361

Pathways: Signalisation MAPK, Signalisation RTK, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Response to Growth Hormone Stimulus, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Protein targeting to Nucleus, Toll-Like Receptors Cascades, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, VEGFR1 Specific Signals, S100 Proteins