PRKACA anticorps

N° du produit ABIN238374

Cet anticorps anti-PRKACA Monoclonal Souris (Clone 6D2-1) (ABIN238374) détecte spécifiquement PRKACA dans WB. L’anticorps est réactif avec des échantillons de Humain.

Aperçu rapide pour PRKACA anticorps (ABIN238374)

Antigène: PRKACA (Protein Kinase A, alpha (PRKACA))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp PRKACA est non-conjugé

Application: Western Blotting (WB)

Clone: 6D2-1

Détail du produit anti-PRKACA anticorps

Specificité: This antibody strongly reacts with human proteinkinase A catalytic (PKAc) alpha subunit, and weakly with PKAc gamma subunit (both around 40 kDa). The recognized epitope of PKAc alpha is identical between man, sheep, pig, ox and dog.

Attributs du produit: Synonyms: PKA C-alpha, cAMP-dependent protein kinase catalytic subunit alpha

Purification: Protein A affinity chromatography.

Immunogène: Peptide corresponding to amino acids ESPAQNTAHLDQFERIK of human proteinkinase A calpha (PKAc alpha).

Isotype: IgG1

Information d'application

Indications d'application: Suitable for Western Blotting (0.5-1 μg/mL). Positive control: HeLa human cervix carcinoma cell lineNote: To detect PKAc gamma, use a more concentrated lysate from a tissue expressing thissubunit (testis).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.4 with 15 mM sodium azide as preservative.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid freeze/thaw cycles.
Shelf life: One year from despatch.

Date de péremption: 12 months

Détails sur PRKACA

Antigène: PRKACA (Protein Kinase A, alpha (PRKACA))

Autre désignation: PRKACA

Sujet: Protein kinase A (PKA, cAMP-dependent protein kinase) is a key element of a ubiquitous signaling pathway important in the cell cycle, cellular communication, memory formation and behavior. PKA is composed of two catalytic (PKAc, Protein Kinase A catalytic subunit) and two regulatory subunits (PKAr). Upon binding cAMP, the complex dissociates to PKAr dimer and two activated PKAc ser/thr protein kinase catalytic monomers. The released PKAc can translocate into the nucleus and exert a regulatory role in the activation of multiple nuclear hormone receptors. However, PKAc-mediated activation of tonicity-dependent gene expression is cAMP independent. Humans express three types of PKAc subunit - PKAc alpha is present in most human tissues, PKAc beta and gamma are tissue-specific, the later is found in testes.Synonyms: PKA C-alpha, cAMP-dependent protein kinase catalytic subunit alpha

ID gène: 5566

UniProt: P17612

Pathways: Signalisation NF-kappaB, Signalisation Hedgehog, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Thyroid Hormone Synthesis, Carbohydrate Homeostasis, Myometrial Relaxation and Contraction, M Phase, G-protein mediated Events, Signaling Events mediated by VEGFR1 and VEGFR2, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, VEGFR1 Specific Signals, Lipid Metabolism, SARS-CoV-2 Protein Interactome, Phosphorylation & l'infection par le SRAS-CoV-2