p53 anticorps (C-Term, pSer392)

N° du produit ABIN238404

Détail du produit anti-p53 anticorps

Antigène: p53 (TP53) (Tumor Protein P53 (TP53))

Épitope: AA 378-393, C-Term, pSer392

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp p53 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificité: This p53 antibody FP3.2 [FPS392] reacts with Human p53 tumour suppressor protein phosphorylated at CKII site (Ser392).

Réactivité croisée (Details): Species reactivity (expected):Rabbit (85 %), Canine (85 %), Guinea pig (85 %), Monkey (92 %), Pig (85 %).
Species reactivity (tested):Human, Mouse.

Attributs du produit: Synonyms: Cellular tumor antigen p53, Tumor suppressor p53, Phosphoprotein p53, NY-CO-13

Purification: Protein A Affinity Chromatography

Immunogène: KLH-conjugated phosphopeptide RHKKLMFKTEGPDS[P] corresponding to amino acids378-393 representing the C-terminus of Human p53.

Clone: FP3-2

Isotype: IgG1

Information d'application

Indications d'application: Western Blotting: 1 μg/mL. Immunohistochemistry on Paraffin Sections): 10 μg/mLStaining technique: Standard ABC technique (DAB+)Pretreatment: High temperature antigen retrieval (microwave, pressure cooker) in 10 mMcitrate buffer pH 6.0 or 1 mM EDTA-NaOH buffer pH 8.0. Incubation Time: 1 hour at RT or overnight at 4 °CPositive Tissue: breast carcinoma with high level of wild-type p53.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: PBS, pH ~7.4, 15 mM Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Date de péremption: 12 months

Détails sur p53

Antigène: p53 (TP53) (Tumor Protein P53 (TP53))

Autre désignation: p53 (TP53) (TP53 Produits)

Synonymes: anticorps BCC7, anticorps LFS1, anticorps P53, anticorps TRP53, anticorps p53, anticorps Trp53, anticorps brp53, anticorps drp53, anticorps etID22686.5, anticorps fb40d06, anticorps wu:fb40d06, anticorps zgc:111919, anticorps Tp53, anticorps bbl, anticorps bfy, anticorps bhy, anticorps p44, anticorps tp53, anticorps Xp53, anticorps TP53, anticorps tumor protein p53, anticorps cellular tumor antigen p53, anticorps transformation related protein 53, anticorps tumor protein p53 L homeolog, anticorps TP53, anticorps CpipJ_CPIJ002758, anticorps Tp53, anticorps tp53, anticorps Trp53, anticorps tp53.L

Sujet: The tumour suppressor protein p53 is a key element of intracellular anticancer protection. It mediates cell cycle arrest or apoptosis in response to DNA damage or to starvation for pyrimidine nukleotides. It is up-regulated in response to these stress signals and stimulated to activate transcription of specific genes, resulting in expression of p21waf1 and other proteins involved in G1 or G2/M arrest, or proteins that trigger apoptosis, such as Bcl-2. The structure of p53 comprises N-terminal transactivation domain, central DNA-binding domain, oligomerisation domain, and C-terminal regulatory domain. There are various phosphorylation sites on p53, of which the phosphorylation at Ser15 is important for p53 activation and stabilization.Synonyms: Cellular tumor antigen p53, NY-CO-13, Phosphoprotein p53, Tumor suppressor p53

ID gène: 7157

UniProt: P04637

Pathways: Signalisation p53, Signalisation MAPK, Signalisation PI3K-Akt, Apoptose, AMPK Signaling, Chromatin Binding, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Autophagy, L'effet Warburg