HLA-DR anticorps

N° du produit ABIN238445

L’anticorps anti-HLA-DR Monoclonal Souris est utilisé pour la détection de HLA-DR dans des échantillons de Humain. Il a été validé pour FACS, WB et IP.

Aperçu rapide pour HLA-DR anticorps (ABIN238445)

Antigène: HLA-DR

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp HLA-DR est non-conjugé

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunoprecipitation (IP)

Clone: MEM-12

Détail du produit anti-HLA-DR anticorps

Réactivité croisée (Details): Species reactivity (tested):Human

Attributs du produit: Synonyms: HLA-DR, HLA class II histocompatibility antigen DR, MHC class II antigen DR

Purification: Affinity Chromatography on Protein A

Immunogène: Thymocyte membrane

Isotype: IgG1

Information d'application

Indications d'application: Flow Cytometry: Positive Control: B-lymphocytes, Monocytes, DAUDI cell line, RAJI cell line. Immunoprecipitation: Positive control: B-lymphocytes, Monocytes, DAUDI cell line, RAJI cell line. Western Blotting (Non-Reducing Conditions): Positive control: B-lymphocytes, Monocytes, DAUDI cell line, RAJI cell line. Sample preparation: Resuspend approx.50 mil. cells in 1 mLcold Lysis buffer (1 % laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Proteaseinhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysatewith non-reducing SDS-PAGE sample buffer. Do not heat/boil.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.4 with 15 mM sodium azide as preservative

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Date de péremption: 12 months

Détails sur HLA-DR

Antigène: HLA-DR

Autre désignation: HLA Class II DR

Sujet: HLA DR, like other MHC class II molecules, is a transmembrane glycoprotein composed of an alpha chain (36 kDa) and a beta chain (27 kDa). It is expressed primarily on antigen presenting cells such as B lymphocytes, monocytes, macrophages, thymic epithelial cells and activated T lymphocytes. Three loci, DR, DQ and DP, encode the major expressed products of the human class II region. The human MHC class II molecules bind intracellularly processed peptides and present them to T helper cells. They therefore have a critical role in the initiation of the immune response.Synonyms: HLA class II histocompatibility antigen DR, HLA-DR, MHC class II antigen DR

Pathways: Human Leukocyte Antigen (HLA) in Adaptive Immune Response