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HLA-DR anticorps (HLA-DR) Primary Antibody

HLA-DR Reactivité: Humain FACS, IF, IHC, ISt, StM, WB Hôte: Souris Monoclonal SPM289
N° du produit ABIN6939641
$508.20
Plus shipping costs $45.00
100 μg ABIN6939641
100 μg ABIN6939641
local_shipping Destination: Etats-Unis
Envoi sous 2 à 4 jours ouvrables
  • Antigène
    Reactivité
    Humain
    Hôte
    Souris
    Clonalité
    Monoclonal
    Conjugué
    Cet anticorp HLA-DR est non-conjugé
    Application
    Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunostaining (ISt), Staining Methods (StM), Western Blotting (WB)
    Specificité
    This MAb reacts with the beta-chain of HLA-DR antigen, a member of MHC class II molecules. It does not cross react with HLA-DP and HLA-DQ. The L243 antibody recognizes a different epitope than the SPM289 monoclonal antibody, and these antibodies do not cross-block binding to each other's respective epitopes. HLA-DR is a heterodimeric cell surface glycoprotein comprised of a 36 kDa alpha (heavy) chain and a 28 kDa beta (light) chain. It is expressed on B-cells, activated T-cells, monocytes/macrophages, dendritic cells and other non-professional APCs. In conjunction with the CD3/TCR complex and CD4 Molecules, HLA-DR is critical for efficient peptide presentation to CD4+ T cells. It is an excellent histiocytic marker in paraffin sections producing intense staining. True histiocytic neoplasms are similarly positive. HLA-DR antigens also occur on a variety of epithelial cells and their corresponding neoplastic counterparts.
    Purification
    Purified by Protein A/G
    Immunogène
    Activated human peripheral blood mononuclear cells
    Clone
    SPM289
    Isotype
    IgG2b, kappa
  • Indications d'application

    Positive Control: Raji, Ramos, Daudi or HuT78 cells. Spleen, Tonsil or lymph node.

    Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (2-4 μg/mL), Western Blot (0.5-2 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

    Restrictions
    For Research Use only
  • Concentration
    200 μg/mL
    Buffer
    10 mM PBS with 0.05 % BSA & 0.05 % azide.
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Stock
    4 °C,-80 °C
    Stockage commentaire
    Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
    Date de péremption
    24 months
  • Antigène
    Autre désignation
    HLA-DR
    Poids moléculaire
    ~28kDa (beta chain)
    ID gène
    3123
    UniProt
    P01903
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