SMAD7
Reactivité: Humain
WB, ELISA, IF
Hôte: Lapin
Polyclonal
unconjugated
Indications d'application
SMAD7 antibody can be used for detection of SMAD7 by ELISA at 1:62500. SMAD7 antibody can be used for detection of SMAD7 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Restrictions
For Research Use only
Format
Lyophilized
Reconstitution
Add 100 ?L of distilled water. Final antibody concentration is 1 mg/mL.
Concentration
1 mg/mL
Buffer
Antibody is lyophilized in PBS buffer with 2 % sucrose.
Conseil sur la manipulation
As with any antibody avoid repeat freeze-thaw cycles.
Stock
4 °C/-20 °C
Stockage commentaire
For short periods of storage (days) store at 4 °C. For longer periods of storage, store SMAD7 antibody at -20 °C.
Antigène
SMAD7
(SMAD, Mothers Against DPP Homolog 7 (SMAD7))
anticorps CRCS3, anticorps MADH7, anticorps MADH8, anticorps Smad7a, anticorps Madh7, anticorps Smad10, anticorps madh7, anticorps xsmad7, anticorps wu:fj60c10, anticorps SMAD family member 7, anticorps SMAD family member 7 L homeolog, anticorps SMAD7, anticorps Smad7, anticorps smad7.L, anticorps smad7
Sujet
Smad7 acts to functionally inactivate RB and de-repress E2F without blocking the activation of TbetaRI and the nuclear translocation of Smad2/3, allowing TGF-beta1 to exert effects in a cancer cell that is resistant to TGF-beta1-mediated growth inhibition.