Zona Pellucida Glycoprotein 3 anticorps

N° du produit ABIN2461230

Détail du produit anti-Zona Pellucida Glycoprotein 3 anticorps

Antigène: Zona Pellucida Glycoprotein 3 (ZP3)

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Zona Pellucida Glycoprotein 3 est non-conjugé

Application: Western Blotting (WB), ELISA

Purification: Antibody is purified by protein A chromatography method.

Immunogène: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human ZP3.

Information d'application

Indications d'application: ZP3 antibody can be used for detection of ZP3 by ELISA at 1:62500. ZP3 antibody can be used for detection of ZP3 by western blot at 0.6 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 100 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Concentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Conseil sur la manipulation: As with any antibody avoid repeat freeze-thaw cycles.

Stock: 4 °C/-20 °C

Stockage commentaire: For short periods of storage (days) store at 4 °C. For longer periods of storage, store ZP3 antibody at -20 °C.

Détails sur Zona Pellucida Glycoprotein 3

Antigène: Zona Pellucida Glycoprotein 3 (ZP3)

Autre désignation: ZP3 (ZP3 Produits)

Synonymes: anticorps ZP3A, anticorps ZP3B, anticorps ZPC, anticorps Zp-3, anticorps zp3.2, anticorps Zp-3B, anticorps zona pellucida glycoprotein 3, anticorps zona pellucida sperm-binding protein 3, anticorps ZP3, anticorps zp3, anticorps Zp3

Sujet: The zona pellucida is an extracellular matrix that surrounds the oocyte and early embryo. It is composed primarily of three or four glycoproteins with various functions during fertilization and preimplantation development. The protein encoded by this gene is a structural component of the zona pellucida and functions in primary binding and induction of the sperm acrosome reaction. The nascent protein contains a N-terminal signal peptide sequence, a conserved ZP domain, a C-terminal consensus furin cleavage site, and a transmembrane domain. It is hypothesized that furin cleavage results in release of the mature protein from the plasma membrane for subsequent incorporation into the zona pellucida matrix. However, the requirement for furin cleavage in this process remains controversial based on mouse studies. A variation in the last exon of this gene has previously served as the basis for an additional ZP3 locus, however, sequence and literature review reveals that there is only one full-length ZP3 locus in the human genome. Another locus encoding a bipartite transcript designated POMZP3 contains a duplication of the last four exons of ZP3, including the above described variation, and maps closely to this gene

Poids moléculaire: 47 kDa, 23 kDa

ID gène: 7784

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process