MBD1 anticorps

N° du produit ABIN2462036

Détail du produit anti-MBD1 anticorps

Antigène: MBD1 (Methyl-CpG Binding Domain Protein 1 (MBD1))

Reactivité: Humain, Chien

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp MBD1 est non-conjugé

Application: Western Blotting (WB), ELISA

Purification: Antibody is purified by protein A chromatography method.

Immunogène: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human MBD1.

Information d'application

Indications d'application: MBD1 antibody can be used for detection of MBD1 by ELISA at 1:312500. MBD1 antibody can be used for detection of MBD1 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 100 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Concentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Conseil sur la manipulation: As with any antibody avoid repeat freeze-thaw cycles.

Stock: 4 °C/-20 °C

Stockage commentaire: For short periods of storage (days) store at 4 °C. For longer periods of storage, store MBD1 antibody at -20 °C.

Détails sur MBD1

Antigène: MBD1 (Methyl-CpG Binding Domain Protein 1 (MBD1))

Autre désignation: MBD1 (MBD1 Produits)

Synonymes: anticorps CXXC3, anticorps PCM1, anticorps RFT, anticorps Cxxc3, anticorps CCDC11, anticorps methyl-CpG binding domain protein 1, anticorps MBD1, anticorps Mbd1

Sujet: MBD1 belongs to a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MBD1 can also repress transcription from methylated gene promoters. Five transcript variants of the MBD1 are generated by alternative splicing resulting in protein isoforms that contain one MBD domain, two to three cysteine-rich (CXXC) domains, and some differences in the COOH terminus. All five transcript variants repress transcription from methylated promoters, in addition, variants with three CXXC domains also repress unmethylated promoter activity. DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MECP2, MBD1 and MBD2 can also repress transcription from methylated gene promoters. Five transcript variants of the MBD1 are generated by alternative splicing resulting in protein isoforms that contain one MBD domain, two to three cysteine-rich (CXXC) domains, and some differences in the COOH terminus. All five transcript variants repress transcription from methylated promoters, in addition, variants with three CXXC domains also repress unmethylated promoter activity. MBD1 and MBD2 map very close to each other on chromosome 18q21.

Poids moléculaire: 60 kDa

ID gène: 4152

NCBI Accession: NP_056669