SF3A1 anticorps

N° du produit ABIN2462195

Détail du produit anti-SF3A1 anticorps

Antigène: SF3A1 (Splicing Factor 3a, Subunit 1 (SF3A1))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp SF3A1 est non-conjugé

Application: Western Blotting (WB), ELISA

Purification: Antibody is purified by peptide affinity chromatography method.

Immunogène: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human SF3A1.

Information d'application

Indications d'application: SF3A1 antibody can be used for detection of SF3A1 by ELISA at 1:62500. SF3A1 antibody can be used for detection of SF3A1 by western blot at 0.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 50 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Concentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Conseil sur la manipulation: As with any antibody avoid repeat freeze-thaw cycles.

Stock: 4 °C/-20 °C

Stockage commentaire: For short periods of storage (days) store at 4 °C. For longer periods of storage, store SF3A1 antibody at -20 °C.

Détails sur SF3A1

Antigène: SF3A1 (Splicing Factor 3a, Subunit 1 (SF3A1))

Autre désignation: SF3A1 (SF3A1 Produits)

Synonymes: anticorps Dmel\\CG16941, anticorps SF3a1, anticorps SF3a120, anticorps DDBDRAFT_0168938, anticorps DDBDRAFT_0233180, anticorps DDB_0168938, anticorps DDB_0233180, anticorps 1200014H24Rik, anticorps 5930416L09Rik, anticorps AI159724, anticorps si:dz150f13.2, anticorps wu:fc38e01, anticorps wu:fc50a11, anticorps wu:fj37c05, anticorps zgc:65786, anticorps PRP21, anticorps PRPF21, anticorps SAP114, anticorps SF3A120, anticorps CG16941 gene product from transcript CG16941-RA, anticorps SWAP/Surp domain-containing protein, anticorps splicing factor 3a, subunit 1, anticorps splicing factor 3a subunit 1, anticorps CG16941, anticorps sf3a1, anticorps spl1, anticorps Sf3a1, anticorps SF3A1

Sujet: SF3A1 is the subunit 1 of the splicing factor 3a protein complex. The splicing factor 3a heterotrimer includes subunits 1, 2 and 3 and is necessary for the in vitro conversion of 15S U2 snRNP into an active 17S particle that performs pre-mRNA splicing. Subunit 1 belongs to the SURP protein family, named for the SURP motifs that are thought to mediate RNA binding. Subunit 1 has tandemly repeated SURP motifs in its amino-terminal half while its carboxy-terminal half contains a proline-rich region and a ubiquitin-like domain. Binding studies with truncated subunit 1 derivatives demonstrated that the two SURP motifs are necessary for binding to subunit 3 while contacts with subunit 2 may occur through sequences carboxy-terminal to the SURP motifs.This gene encodes subunit 1 of the splicing factor 3a protein complex. The splicing factor 3a heterotrimer includes subunits 1, 2 and 3 and is necessary for the in vitro conversion of 15S U2 snRNP into an active 17S particle that performs pre-mRNA splicing. Subunit 1 belongs to the SURP protein family, named for the SURP (also called SWAP or Suppressor-of-White-APricot) motifs that are thought to mediate RNA binding. Subunit 1 has tandemly repeated SURP motifs in its amino-terminal half while its carboxy-terminal half contains a proline-rich region and a ubiquitin-like domain. Binding studies with truncated subunit 1 derivatives demonstrated that the two SURP motifs are necessary for binding to subunit 3 while contacts with subunit 2 may occur through sequences carboxy-terminal to the SURP motifs. Alternative splicing results in multiple transcript variants encoding different isoforms.

Poids moléculaire: 89 kDa

ID gène: 10291

NCBI Accession: NP_005868

UniProt: Q15459

Pathways: Ribonucleoprotein Complex Subunit Organization