Western Blotting (WB), Immunohistochemistry (IHC), ELISA
Purification
p70 S6 Kinase antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogène
p70 S6 Kinase antibody was raised against a 13 amino acid synthetic peptide near the C-Terminus of p70 S6 Kinase.
RPS6KB1
Reactivité: Humain, Souris, Rat, Porc
WB, IHC, ELISA, IF, ICC
Hôte: Lapin
Polyclonal
unconjugated
Indications d'application
ELISA: Antibody detection limit dilution >1:128,000. Western Blot: Approximately 65-70 kDa band observed in human muscle and placenta lysates (predicted MW of 60 kDa according to NP_003152.1). Recommended for use at 0.2-1 μg/mL. Immunohistochemistry: In paraffin embedded human kidney shows ribosomal staining in DCT cells. Recommended concentration, 1-2 μg/mL.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Conseil sur la manipulation
Minimize freezing and thawing.
Stock
-20 °C
Stockage commentaire
Aliquot and store at -20 °C.
Antigène
RPS6KB1
(Ribosomal Protein S6 Kinase, 70kDa, Polypeptide 1 (RPS6KB1))