ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
Specificité
This antibody is expected to recognize isoforms 1, 2 and 3 (NP_671700.1, NP_005742.4, NP_671714.1 respectively). The isoforms Yotiao, AKAP350B and AKAP350C are not recognized.
Purification
AKAP9 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogène
AKAP9 antibody was raised against a 12 amino acid synthetic peptide near the C-Terminus of AKAP9.
AKAP9
Reactivité: Humain
ELISA
Hôte: Lapin
Polyclonal
Biotin
Indications d'application
ELISA: Antibody detection limit dilution 1:32,000. Western Blot: Approximately 450 kDa band observed in HEPG2 lysate (calculated MW of 454 kDa according to NP_ 671700.1). Recommended concentration: 1-3 μg/mL.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Conseil sur la manipulation
Minimize freezing and thawing.
Stock
-20 °C
Stockage commentaire
Aliquot and store at -20 °C.
Antigène
AKAP9
(A Kinase (PRKA) Anchor Protein (Yotiao) 9 (AKAP9))