Western Blotting (WB), Immunohistochemistry (IHC), ELISA
Purification
CARD11 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogène
CARD11 antibody was raised against a 12 amino acid synthetic peptide near the C-Terminus of CARD11.
ELISA: Antibody detection limit dilution 1:32000. Western Blot: Approximately 150+65 kDa bands observed in Jurkat lysate (calculated MW of 133 kDa according to NP_115791.2). A similar pattern was observed in HEK293 transiently expressing CARD11 (pciture not shown). These bands are not observed in the non-transfected HEK293. Recommended concentration: 1-3 μg/mL. an additional band of unknown identity was also consistently observed at 60 kDa . This band was successfully blocked by incubation with the immunizing peptide. Immunohistochemistry: In paraffin embedded human spleen shows nuclear staining. Recommended concentration, 2-5 μg/mL.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Conseil sur la manipulation
Minimize freezing and thawing.
Stock
-20 °C
Stockage commentaire
Aliquot and store at -20 °C.
Antigène
CARD11
(Caspase Recruitment Domain Family, Member 11 (CARD11))