Cet anticorp Serotonin Receptor 3A est non-conjugé
Application
Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)
Specificité
This antibody is expected to recognize both reported isoforms (NP_998786.1 and NP_000860.1).
Purification
Serotonin receptor 3A antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogène
Serotonin receptor 3A antibody was raised against a 12 amino acid synthetic peptide near the internal region of Serotonin receptor 3A.
HTR3A
Reactivité: Humain, Souris, Rat, Chien, Poulet, Hamster, Singe
WB
Hôte: Lapin
Polyclonal
unconjugated
Indications d'application
ELISA: Antibody detection limit dilution 1:128,000. Western Blot: Approximately 55 kDa band observed in human colon lysates (calculated MW of 55.3 kDa according to NP_000860.1. Recommended concentration: 0.3-1 μg/mL. A minor band of unknown identity was also consistently observed at 200 kDa . This band was successfully blocked by incubation with the immunizing peptide.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.