This antibody specifically recognises an epitope within the C-terminal (CT) region of endothelin B receptor (ET-B), a widely distributed transmembrane protein and member of the G-protein coupled receptor 1 family, which acts as a non-specific receptor for endothelin 1, 2, and 3, and mediates its action by G protein association and activation of a phosphatidylinositol-calcium second messenger system. This antibody recognizes epitopes on the cytoplasmic side of the receptor and therefore the cell membrane must be permeable to the antibody. This antibody does not block the binding of endothelins. This antibody is reported as suitable for use in immunocytochemistry on acetone fixed cells.
Réactivité croisée
Humain, Souris
Attributs du produit
Purified IgG
Purification
Purified
Immunogène
Keyhole limpet haemocyanin (KLH) conjugated C-terminal peptide KANDHGYDNFRSSNN corresponding to amino acids 424 to 437 of rat endothelin B receptor.