ATP6AP1 anticorps

N° du produit ABIN249357

Détail du produit anti-ATP6AP1 anticorps

Antigène: ATP6AP1 (ATPase, H+ Transporting, Lysosomal Accessory Protein 1 (ATP6AP1))

Reactivité: Arabidopsis thaliana, Chlamydomonas, Soybean

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp ATP6AP1 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunolocalization (IL)

Réactivité croisée: Soja (Glycine max)

Réactivité croisée (Details): No cross-reactivity with: Aspergillus niger

Attributs du produit: Expected / apparent Molecular Weight of the Antigene: 95 kDa (Arabidopsis thaliana)

Purification: serum

Immunogène: KLH-conjugated synthetic peptide derived from available di and monocot, fern, mosses and algal plasma membrane ATPase sequences including Arabidopsis thaliana ATPase 1 (At2g18960) and ATPase 2,3,4,6,7,8,9 of Arabidopsis thaliana and hydrogen ATPase of Chlamydomonas reinhardtii (Q9FNS3)

Information d'application

Indications d'application: Recommended Dilution: 1 : 1000 - 1 : 5000 with standard ECL OR 1 : 10 000 with ECL-Advance, enhanced chemiluminescence (WB), 1 : 600 - 1 : 1000 (IF).
Cellular [compartment marker] for plasma membrane

Commentaires:

for additional Western blot detection image please refer to the article below.VERY IMPORTANT: please, do not heat up your samples over 70°C as this might cause H+ATPase to precipitate and there will be no signal on your western blot.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: For reconstitution add 200 µL of sterile water.

Buffer: PBS pH 7.4

Conseil sur la manipulation: Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Once reconstituted make aliquots to avoid repreated freeze-thaw cycles.

Stock: -20 °C

Stockage commentaire: Do not store this antibody at 4 °C.

Références pour anticorps anti-ATP6AP1 (ABIN249357)

Sobrino-Plata, Carrasco-Gil, Abadía, Escobar, Álvarez-Fernández, Hernández: "The role of glutathione in mercury tolerance resembles its function under cadmium stress in Arabidopsis." dans: Metallomics : integrated biometal science, Vol. 6, Issue 2, pp. 356-66, (2014) (PubMed).

Chen, Fujii, Yamaji, Masuda, Takemoto, Kamiya, Yusuyin, Iwasaki, Kato, Maeshima, Ma, Ueno: "Mn tolerance in rice is mediated by MTP8.1, a member of the cation diffusion facilitator family." dans: Journal of experimental botany, Vol. 64, Issue 14, pp. 4375-87, (2013) (PubMed).

Menckhoff, Mielke-Ehret, Buck, Vuletić, Lüthje: "Plasma membrane-associated malate dehydrogenase of maize (Zea mays L.) roots: Native versus recombinant protein." dans: Journal of proteomics, Vol. 80C, pp. 66-77, (2013) (PubMed).

Visnovitz, Solti, Csikós, Fricke: "Plasma membrane H(+) -ATPase gene expression, protein level and activity in growing and non-growing regions of barley (Hordeum vulgare) leaves." dans: Physiologia plantarum, Vol. 144, Issue 4, pp. 382-93, (2012) (PubMed).

Zhang, Peck: "Simplified enrichment of plasma membrane proteins for proteomic analyses in Arabidopsis thaliana." dans: Proteomics, Vol. 11, Issue 9, pp. 1780-8, (2011) (PubMed).

Ito, Batth, Petzold, Redding-Johanson, Mukhopadhyay, Verboom, Meyer, Millar, Heazlewood: "Analysis of the Arabidopsis Cytosolic Proteome Highlights Subcellular Partitioning of Central Plant Metabolism." dans: Journal of proteome research, (2010) (PubMed).

Chen, Xiao, Wu, Dong, He, Pei, Zheng: "Nitric oxide enhances salt secretion and Na(+) sequestration in a mangrove plant, Avicennia marina, through increasing the expression of H(+)-ATPase and Na(+)/H(+) antiporter under high salinity." dans: Tree physiology, Vol. 30, Issue 12, pp. 1570-85, (2010) (PubMed).

Page, Kropat, Hamel, Merchant: "Two Chlamydomonas CTR copper transporters with a novel cys-met motif are localized to the plasma membrane and function in copper assimilation." dans: The Plant cell, Vol. 21, Issue 3, pp. 928-43, (2009) (PubMed).

Détails sur ATP6AP1

Antigène: ATP6AP1 (ATPase, H+ Transporting, Lysosomal Accessory Protein 1 (ATP6AP1))

Autre désignation: H+ATPase (ATP6AP1 Produits)

Synonymes: anticorps 16A, anticorps ATP6IP1, anticorps ATP6S1, anticorps Ac45, anticorps CF2, anticorps VATPS1, anticorps XAP-3, anticorps XAP3, anticorps AC45, anticorps AI316502, anticorps AW108110, anticorps Atp6ip1, anticorps Atp6s1, anticorps C7-1, anticorps mFLJ00383, anticorps H+-ATPase, anticorps ATPase H+ transporting accessory protein 1, anticorps ATPase, H+ transporting, lysosomal accessory protein 1, anticorps plasma membrane ATPase 4, anticorps ATP6AP1, anticorps Atp6ap1, anticorps LOC547525

Sujet: AGI Code: At2g18960
The Plasma Membrane H+ATPase is a family of proteins of ca. 100 kDa that are believed to be exclusive to the plasma membranes of plants and fungi. The protein is anchored within biological membrane which creates an electrochemical gradient used as an energy source and is essential for uptake of most metabolites and plant responses to environment, for example movement of leaves.

Poids moléculaire: 95 kDa (Arabidopsis thaliana)

UniProt: P20649

Pathways: Proton Transport, SARS-CoV-2 Protein Interactome