PsbO anticorps

N° du produit ABIN2559392

Détail du produit

Antigène: PsbO

Reactivité: Spinach, Arabidopsis thaliana, Orge, Chlamydomonas reinhardtii

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Inconjugué

Application: Western Blotting (WB)

Homologie: dicots including: Nicotiana tabacum, Solanum lycopersicum, trees: Populus trichocarpa

Attributs du produit: Expected / apparent Molecular Weight of the Antigene: 35 / 33 kDa

Purification: affinity purified

Immunogène: native purified 33 kDa protein from Spinacia oleracea UniProt P12359

Information d'application

Indications d'application: Recommended Dilution 1 : 2000 - 1 : 5000 with standard ECL (WB).

Commentaires:

This antibody can be used as a loading control for Chlamydomonas reinhardtii while it not so suitable for higher plants as accumulation of these proteins might drop to 12.5-25 % of the WT level in mutants defective for PSII core (Schult et al. 2007).

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Buffer: PBS pH 7.4

Conseil sur la manipulation: Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Once reconstituted make aliquots to avoid repreated freeze-thaw cycles.

Stock: -20 °C

Référence

Lin, Liu, Kuo, Tsai, Feng, Huang, Chien: "Knockdown of PsbO leads to induction of HydA and production of photobiological H2 in the green alga Chlorella sp. DT." dans: Bioresource technology, Vol. 143, pp. 154-62, (2013) (PubMed).

Détail du antigène

Antigène: PsbO

Sujet: PSII reaction centre components are generating the redox potential required to drive highly oxidizing water splitting reaction. Four Mn atoms are present on a lumenal surface and form the catalyctic site of the water-splitting reaction which is in close association with the 33 kDa (PsbO), 23 kDa (PsbP) and 17 kDa (PsbQ) extrinistic subunits of oxygen evolving complex OEC. A 33-kDa extrinsic protein is also termed the Mn-stabilizing protein (MSP), however recent evidences shown that it is C-terminal domain of PsbA (D1) protein which is involved in in the assembly and stabilization of the OEC.

Poids moléculaire: expected: 35 kDa, apparent: 33 kDa