p53 anticorps (AA 371-380)

N° du produit ABIN264449

L’anticorps Souris Monoclonal anti-p53 a été validé pour WB, IP, IHC (fro) et EIA. Il convient pour détecter p53 dans des échantillons de Humain, Souris, Rat et Singe.

Aperçu rapide pour p53 anticorps (AA 371-380) (ABIN264449)

Antigène: p53 (TP53) (Tumor Protein P53 (TP53))

Reactivité: Humain, Souris, Rat, Singe

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp p53 est non-conjugé

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Clone: HR231

Détail du produit anti-p53 anticorps

Épitope: AA 371-380

Specificité: This antibody reacts to p53.

Réactivité croisée (Details): Species reactivity (tested):Human, Monkey, Mouse and Rat.

Attributs du produit: Synonyms: Cellular tumor antigen p53, Tumor suppressor p53, Phosphoprotein p53, NY-CO-13

Purification: Affinity chromatography on Protein A/G

Immunogène: Hybridoma produced by the fusion of splenocytes from immunized with full length humanp53 protein and mouse myeloma cells.

Isotype: IgG2b

Information d'application

Indications d'application: Western Blot (1-5 μg/mL). ELISA. Immunoprecipitation. Immunohistochemistry. Positive Control: Breast or colon carcinoma cells.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1,0 mg/mL

Buffer: PBS, 0.08 % Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store the antibody at -20 °C. Avoid repeated freezing and thawing.
Shelf life: One year from despatch.

Date de péremption: 12 months

Détails sur p53

Antigène: p53 (TP53) (Tumor Protein P53 (TP53))

Autre désignation: p53 (TP53)

Sujet: P53 is a 53 kDa transcription factor that can inhibit cell cycle progression or induce apoptosis in response to stress or DNA damage. Disruption of the p53 signalling pathway through various mechanisms is the most common alteration in human cancer occuring in over half of all tumors. The p53 protein is short lived and expressed at low levels in normal cells but accumulates and/or is activated in cells that have undergone genotoxic damage or oncogene activation. Many tumor derived and transformed cell lines express elevated levels of mutant p53 protein. Other genes also implicated in the downstream effects as a result of p53 activation are: p21WAF1, GADD45, 14-3-3, bax, Fas/APO1, KILLER/ DR5, Tsp1, IGF-BP3 and others.Synonyms: Cellular tumor antigen p53, NY-CO-13, Phosphoprotein p53, Tumor suppressor p53

ID gène: 7157

UniProt: P04637

Pathways: Signalisation p53, Signalisation MAPK, Signalisation PI3K-Akt, Apoptose, AMPK Signaling, Chromatin Binding, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Autophagy, L'effet Warburg