EEF2K anticorps

N° du produit ABIN265393

Détail du produit anti-EEF2K anticorps

Antigène: EEF2K (Eukaryotic Elongation Factor-2 Kinase (EEF2K))

Reactivité: Humain, Rat, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp EEF2K est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificité: This antibody detects endogenous levels of EEF-2 kinase / eEF2K protein (region surrounding Pro360).

Réactivité croisée (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity chromatography using epitope-specific immunogen

Pureté: > 95 % pure by SDS-PAGE

Information d'application

Indications d'application: ELISA: 1: 5000approx. 1: 20000. WB: 1: 500approx. 1: 1000. IHC: 1: 50approx. 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 15 mM sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détails sur EEF2K

Antigène: EEF2K (Eukaryotic Elongation Factor-2 Kinase (EEF2K))

Autre désignation: EEF-2 Kinase (EEF2K Produits)

Synonymes: anticorps HSU93850, anticorps eEF-2K, anticorps C86191, anticorps SMEF2K, anticorps eEF-2 kinase, anticorps cb365, anticorps fa04b08, anticorps wu:fa04b08, anticorps zgc:100973, anticorps eukaryotic elongation factor 2 kinase, anticorps eukaryotic elongation factor-2 kinase, anticorps EEF2K, anticorps Eef2k, anticorps eef2k, anticorps LOC101930123

Sujet: The activity of the purified eukaryotic elongation-factor-2 kinase (eEF2K) is completely dependent on calcium and calmodulin, and autophosphorylation on serine and threonine residues is calcium/calmodulin-dependent. eEF2K is a ubiquitous protein kinase that phosphorylates and inactivates eEF2, and thus can modulate the rate of polypeptide chain elongation during translation. eEF2K is 120 kDa protein that is detected in skeletal muscle extracts and is phosphorylated rapidly by SAPK4, but poorly by p38, p38g, JNK or ERK 2. SAPK4 phosphorylates eEF2K at Ser 359 and Ser 396 in vitro, causing its inactivation. The phosphorylation of eEF2K at Ser 359 is also induced by insulin-like growth factor-1. Ser 359 is in close proximity to Ser 366 and the Ser 366 residue also becomes phosphorylated in response to growth factors. eEF2K is phosphorylated by p70 S6 kinase at Ser 366 and this results in the inactivation of eEF2K, especially at low (micromolar) calcium concentrations.Synonyms: Calcium/calmodulin-dependent eukaryotic elongation factor 2 kinase, EEF2K, EF2K, Elongation factor 2 kinase

Poids moléculaire: approx. 105 kDa

ID gène: 29904

NCBI Accession: NP_037434

UniProt: O00418

Pathways: AMPK Signaling