Notch1 anticorps (PE)

N° du produit ABIN2663856

Détail du produit anti-Notch1 anticorps (PE)

Antigène: Notch1 (NOTCH1) (Notch 1 (NOTCH1))

Reactivité: Humain, Souris

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Notch1 est conjugé à/à la PE

Application: Flow Cytometry (FACS)

Purification: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.

Clone: MN1A

Isotype: IgG1 kappa

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Concentration: 0.2 mg/mL

Buffer: Phosphate-buffered solution, pH 7.2, containing 0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Protect from prolonged exposure to light. Do not freeze.

Stock: 4 °C

Stockage commentaire: The antibody solution should be stored undiluted between 2°C and 8°C.

Détails sur Notch1

Antigène: Notch1 (NOTCH1) (Notch 1 (NOTCH1))

Autre désignation: Notch 1 (NOTCH1 Produits)

Synonymes: anticorps notch1, anticorps NOTCH1, anticorps hn1, anticorps tan1, anticorps notch, anticorps xotch, anticorps xnotch, anticorps notch-1, anticorps xnotch1, anticorps TAN1, anticorps hN1, anticorps 9930111A19Rik, anticorps Mis6, anticorps N1, anticorps Tan1, anticorps lin-12, anticorps NOTCH, anticorps Xotch, anticorps notch1-a, anticorps notch 1, anticorps fibropellin-1, anticorps Notch homolog 1, translocation-associated (Drosophila), anticorps Notch homolog 1, translocation-associated, anticorps notch 1 S homeolog, anticorps notch1, anticorps LOC100631526, anticorps NOTCH1, anticorps Notch1, anticorps notch1.S

Sujet: Notch1, also known as neurogenic locus notch homolog protein 1, is a large >270 kD protein that functions as a receptor for the membrane ligands Jagged1, Jagged2 and Delta1 to regulate cell fate decisions. Upon ligand activation, the transmembrane Notch1 receptor is cleaved by TNF-alpha converting enzyme (TACE) to produce a membrane-associated intermediate fragment (NEXT). This fragment is further cleaved by presenilin-dependent gamma secretase to release a notch-derived peptide containing the intracellular fragment from the membrane. The released Notch intracellular fragment (NICD) translocates to the nucleus and forms a transcriptional complex with the RBP-J κ transcriptional activator complex to alter differentiation, proliferation, and apoptotic programs. Notch 1 is highly expressed in the brain, lung, and thymus (CD4-CD8- cells and CD4-CD8+ cells) with lower levels of expression observed in the spleen, bone marrow, spinal cord, eyes, mammary gland, liver, intestine, kidney and heart. The transmembrane Notch protein is a heterodimeric protein consisting of a C-terminal fragment and N-terminal fragment (probably linked by disulphide bonds) containing 5 ankyrin repeats, 36 EGF repeats, and 3 Notch/Lin repeats. Notch1 can be modified by phosphorylation. The mN1A monoclonal antibody reacts with the intracellular domain of mouse and human Notch1 and has been reported to have highest affinity for activated intracellular Notch1 and lower affinity for full-length unprocessed/heterodimeric Notch1 forms. This antibody does not recognize rat Notch1 or cross-react with Notch2, 3, or 4.

Pathways: Signalisation Notch, Stem Cell Maintenance, Regulation of Muscle Cell Differentiation, Tube Formation, Skeletal Muscle Fiber Development