Monoclonal antibody hIL-7R-M21 reacts with the 60-90 kDa glycoprotein, CD127. CD127 is also known as the IL-7 receptor alpha (IL-7Rα) subunit. The IL-7 receptor complex is a heterodimer composed of CD127 and the common gamma chain (γc, CD132), shared by other cytokine receptors (IL-2R, IL-4R, IL-9R, IL-15R, and IL-21R). CD127 is expressed on thymocytes, T- and B-cell progenitors, mature T cells, and some lymphoid and myeloid cells. In vitro experiments show the expression of CD127 is down-regulated following T cell activation. Studies indicate that the IL-7 Receptor plays an important role in the proliferation and differentiation of mature T cells. Recently, it has been shown that low surface expression of CD127, in combination with intermediate to high surface expression of CD25, the α chain of the IL-2 receptor complex, can distinguish between human regulatory and conventional CD4+ T cells in human adult and cord blood, lymph nodes and thymus. Profile of CD127 (hIL-7R-M21) expression on peripheral blood lymphocytes. Human blood cells were stained with Biotin-anti CD127 (solid line) or Biotin mouse IgG1 isotype control (dashed line, Cat. No. 555747) followed by Streptavidin-PE (Cat. No. 554061). Erythrocytes were lysed using BD PharmLyse™ lysis buffer (Cat. No. 555899). Flow cytometry was performed on a BD FACSCalibur™ flow cytometry system.
IL7R
Reactivité: Humain
FACS, WB, ELISA, IF (cc), IF (p), IHC (p), IHC (fro)
Hôte: Lapin
Polyclonal
unconjugated
Indications d'application
Optimal working dilution should be determined by the investigator.
Volume d'échantillon
20 μL
Restrictions
For Research Use only
Buffer
Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Conseil sur la manipulation
The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.
Stock
4 °C
Stockage commentaire
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
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