IL7R anticorps (Biotin)

N° du produit ABIN2688949

Détail du produit anti-IL7R anticorps (Biotin)

Antigène: IL7R (Interleukin 7 Receptor (IL7R))

Reactivité: Souris

Hôte: Rat

Clonalité: Monoclonal

Conjugué: Cet anticorp IL7R est conjugé à/à la Biotin

Application: Flow Cytometry (FACS)

Marque: BD Pharmingen™

Attributs du produit: The B12-1 monoclonal antibody reacts with CD127, the 65-75- kDa type I transmembrane protein IL- 7Rα. The high affinity IL-7 receptor complex is composed of at least two transmembrane proteins, IL-7Rα and CD132, the common γ chain. CD127 has some sequence homology to the cytokine receptor superfamily (also known as the hematopoietin receptor superfamily). Interaction between IL-7 and its receptor is important for the proliferation of pre-B lymphocytes and can also trigger proliferation of CD4-CD8- immature thymocytes, as well as mature T cells in the periphery. Mice lacking CD127 display profoundly impaired development of the B and T lymphoid cell lineages, but display no obvious non-lymphoid abnormalities.5 IL-7Rα is expressed on common lymphoid progenitors and early stages of B lineage development in the bone marrow, on the earliest thymocyte progenitors and CD4-CD8- double-negative and CD4+ and CD8+ singlepositive thymocytes, and on most peripheral T lymphocytes. Intestinal intraepithelial lymphocytes with low-density γδ TCR upregulate CD127 expression in response to IL-2, which may be secreted by neighboring αβ TCR-bearing T cells. Expression of CD127 in the bone marrow and spleen. C57BL/6 bone marrow leukocytes (Left panels) and splenocytes (Right panels) were stained with either biotinylated rat IgG2a, κ isotype control mAb R35- 95 (Cat. no. 553928, Top panels) or biotinylated B12-1 antibody (Bottom panels) and either FITC-conjugated RA3-6B2 anti-mouse CD45R/B220, Cat. no. 553087/553088, Left panels) or FITC- conjugated 145-2C11 mAb (anti-mouse CD3e, Cat. no. 553061/553062, Right panels), followed by Streptavidin-PE (Cat. no. 554061, all panels). Flow cytometry was performed on a FACScan .

BD Pharmingen™ Biotin Rat Anti-Mouse CD127 - Biotin - Clone B12-1 - Isotype Rat IgG2a, λ - Reactivity Ms - 0.5 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Mouse CD127-human IgG fusion protein

Clone: B12

Isotype: IgG2a lambda

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.

Stock: 4 °C

Stockage commentaire: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Références pour anticorps anti-IL7R (Biotin) (ABIN2688949)

Akashi, Kondo, Weissman: "Role of interleukin-7 in T-cell development from hematopoietic stem cells." dans: Immunological reviews, Vol. 165, pp. 13-28, (1999) (PubMed).

Fujihashi, Kawabata, Hiroi, Yamamoto, McGhee, Nishikawa, Kiyono: "Interleukin 2 (IL-2) and interleukin 7 (IL-7) reciprocally induce IL-7 and IL-2 receptors on gamma delta T-cell receptor-positive intraepithelial lymphocytes." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 93, Issue 8, pp. 3613-8, (1996) (PubMed).

Noguchi, Nakamura, Russell, Ziegler, Tsang, Cao, Leonard: "Interleukin-2 receptor gamma chain: a functional component of the interleukin-7 receptor." dans: Science (New York, N.Y.), Vol. 262, Issue 5141, pp. 1877-80, (1994) (PubMed).

Peschon, Morrissey, Grabstein, Ramsdell, Maraskovsky, Gliniak, Park, Ziegler, Williams, Ware, Meyer, Davison: "Early lymphocyte expansion is severely impaired in interleukin 7 receptor-deficient mice." dans: The Journal of experimental medicine, Vol. 180, Issue 5, pp. 1955-60, (1994) (PubMed).

Faust, Saffran, Toksoz, Williams, Witte: "Distinctive growth requirements and gene expression patterns distinguish progenitor B cells from pre-B cells." dans: The Journal of experimental medicine, Vol. 177, Issue 4, pp. 915-23, (1993) (PubMed).

Sudo, Nishikawa, Ohno, Akiyama, Tamakoshi, Yoshida, Nishikawa: "Expression and function of the interleukin 7 receptor in murine lymphocytes." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 90, Issue 19, pp. 9125-9, (1993) (PubMed).

Henderson, Narayanan, Collins, Dorshkind: "Status of kappa L chain gene rearrangements and c-kit and IL-7 receptor expression in stromal cell-dependent pre-B cells." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 149, Issue 6, pp. 1973-9, (1992) (PubMed).

Goodwin, Friend, Ziegler, Jerzy, Falk, Gimpel, Cosman, Dower, March, Namen: "Cloning of the human and murine interleukin-7 receptors: demonstration of a soluble form and homology to a new receptor superfamily." dans: Cell, Vol. 60, Issue 6, pp. 941-51, (1990) (PubMed).

Détails sur IL7R

Antigène: IL7R (Interleukin 7 Receptor (IL7R))

Autre désignation: CD127 (IL7R Produits)

Synonymes: anticorps IL7R, anticorps CD127, anticorps IL-7Ralpha, anticorps CDW127, anticorps IL-7R-alpha, anticorps IL7RA, anticorps ILRA, anticorps IL-7RA, anticorps interleukin 7 receptor, anticorps IL7R, anticorps Il7r

Sujet: Synonyms: IL-7 Receptor α chain

Pathways: Signalistation JAK/STAT, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, Regulation of Cell Size