FAS anticorps

N° du produit ABIN2689460

Détail du produit anti-FAS anticorps

Antigène: FAS (TNF Receptor Superfamily, Member 6 (FAS))

Reactivité: Humain, Porc, Macaque rhésus, Chien, Babouin, Cynomolgus

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp FAS est non-conjugé

Application: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))

Marque: BD Pharmingen™

Attributs du produit: Reacts with the 45 kDa transmembrane cell surface Fas or Apo-1 antigen (designated CD95 at the Fifth HLDA Workshop) which is expressed on a variety of normal and neoplastic cells. The Fas/Apo-1 antigen is a polypeptide, which plays a role in the programmed sequence of events leading to cell death, termed apoptosis. The DX2 clone specifically reacts with murine L cells, murine L1210 leukemia cells and murine P815 mastocytoma cells transfected with human Fas cDNA but not with untransfected parental cell lines. Cross-linking with DX2 delivers an apoptotic signal indicating that DX2 recognizes a functional epitope of the CD95 antigen. This antibody is routinely tested by flow cytometric analysis. Other applications were tested during antibody development only or reported in the literature. Profile of peripheral blood lymphocytes analyzed by flow cytometry DX2-induced apoptosis in Fas transfectant cells line P825/FASDT151 detected by DNA fragmentation analysis. Cells were incubated alone (lane 1), in the presence of mouse IgG1 (lane 2) or in the presence of DX2 at 10, 100 or 500 ng/mL (lanes 3, 4 and 5, respectively).

BD Pharmingen™ Purified Mouse Anti-Human CD95 - Purified - Clone DX2 - Isotype Mouse IgG1, κ - Reactivity Hu, Bab, Cyno, Dog, Pig, Rhe - 0.1 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Clone: DX2

Isotype: IgG1 kappa

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Store undiluted at 4°C.

Références pour anticorps anti-FAS (ABIN2689460)

Cifone, De Maria, Roncaioli, Rippo, Azuma, Lanier, Santoni, Testi: "Apoptotic signaling through CD95 (Fas/Apo-1) activates an acidic sphingomyelinase." dans: The Journal of experimental medicine, Vol. 180, Issue 4, pp. 1547-52, (1994) (PubMed).

Itoh, Yonehara, Ishii, Yonehara, Mizushima, Sameshima, Hase, Seto, Nagata: "The polypeptide encoded by the cDNA for human cell surface antigen Fas can mediate apoptosis." dans: Cell, Vol. 66, Issue 2, pp. 233-43, (1991) (PubMed).

Détails sur FAS

Antigène: FAS (TNF Receptor Superfamily, Member 6 (FAS))

Autre désignation: CD95 (FAS Produits)

Synonymes: anticorps ALPS1A, anticorps APO-1, anticorps APT1, anticorps CD95, anticorps FAS1, anticorps FASTM, anticorps TNFRSF6, anticorps AI196731, anticorps APO1, anticorps TNFR6, anticorps Tnfrsf6, anticorps lpr, anticorps Fas cell surface death receptor, anticorps Fas (TNF receptor superfamily member 6), anticorps FAS, anticorps Fas, anticorps fas

Sujet: Synonyms: Fas/APO-1

Pathways: Signalisation p53, Apoptose, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity