p53 anticorps (Tumor Protein P53) (Alexa Fluor 647)

Details for Product anti-TP53 Antibody No. ABIN2689872
Antigène
  • BCC7
  • LFS1
  • P53
  • TRP53
  • p53
  • Trp53
  • brp53
  • drp53
  • etID22686.5
  • fb40d06
  • wu:fb40d06
  • zgc:111919
  • Tp53
  • bbl
  • bfy
  • bhy
  • p44
  • tp53
  • Xp53
  • TP53
  • tumor protein p53
  • cellular tumor antigen p53
  • transformation related protein 53
  • tumor protein p53 L homeolog
  • TP53
  • CpipJ_CPIJ002758
  • Tp53
  • tp53
  • Trp53
  • tp53.L
Reactivité
Humain
2345
501
466
249
100
64
63
32
26
22
22
17
16
7
6
6
5
4
4
4
3
3
3
2
2
1
1
1
Hôte
Souris
1648
770
7
5
1
1
1
1
Clonalité (Clone)
Monoclonal ()
Conjugué
Cet anticorp p53 est conjugé à/à la Alexa Fluor 647
89
62
48
41
40
40
40
39
39
38
33
32
14
12
12
12
12
11
8
7
7
7
7
7
7
7
7
7
7
7
7
7
7
7
7
7
7
7
7
7
6
5
2
2
2
Application
Intracellular Staining (ICS)
1836
766
747
574
436
413
370
155
109
103
99
89
75
41
32
16
15
15
13
3
2
2
1
1
1
1
1
1
1
1
1
1
Options
Marque BD Phosflow™
Immunogène Human p53 acetylated Peptide
Clone L82
Isotype IgG1, kappa
Attributs du produit The p53 protein is critical to regulation of normal cell growth and proliferation and is a suppressor of tumor cell proliferation. Inactivation of p53 by a number of mechanisms, such as missense mutations or interaction with oncogenic viral or cellular proteins, can result in tumor progression. Mutations and/or allelic loss of the p53 gene are associated with a wide variety of human tumors. Known to have a role in transcriptional regulation, p53 suppresses various promoters containing TATA elements in an apparently sequence-independent fashion. p53 also binds to DNA in a sequence-specific manner via recognition of a 20-bp consensus-binding site. This interaction stimulates the expression of genes downstream of the p53 binding site. A number of genes that contain p53-binding sites have been identified, including MDM2, GADD45, and muscle creatine kinase. Post-translational acetylation of p53 enhances its DNA-binding activity. There are multiple factors that affect p53 acetylation, thereby modulating cellular proliferation and apoptosis. The L82-51 monoclonal antibody recognizes acetylated lysine 382 (acK382) in the C-terminal region of p53. Analysis of p53 (acK382) in lymphocytes. Human peripheral blood mononuclear cells (PBMC) were either treated with 0.4 μM Trichostatin A (Sigma, Cat. No. T8552) plus 0.4 μM Adriamycin (Doxorubicin hydrochloride, Sigma, Cat. No. D1515) for 24 hours (shaded histogram) or untreated (open histogram). The cells were fixed (BD Cytofix™ buffer, Cat. No. 554655) for 10 minutes, then permeabilized (BD Phosflow™ Perm Buffer III, Cat. No. 558050) on ice for at least 30 minutes, and then stained with Alexa Fluor® 647 Mouse anti-p53 (acK382). Lymphocytes were selected by scatter profile. Flow cytometry was performed on a BD FACSArray™ bioanalyzer system. The specificity of mAb L82-51 was confirmed by western blot analysis using unconjugated antibody on lysates from control (left blot) and Trichostatin A-plus-Adriamycin-treated (right blot) PBMC. p53 (acK382) is identified as a band of 53 kDa in the treated cells.

BD Phosflow™ Alexa Fluor® 647 Mouse anti-p53 (acK382) - Alexa Fluor® 647 - Clone L82-51 - Isotype Mouse IgG1, κ - Reactivity Hu - 50 Tests
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Plasmids, Primers & others Plasmids, Primers & others p53 products on genomics-online (e.g. as negative or positive controls)
Antigène
Autre désignation p53 (TP53 Antibody Extrait)
Sujet Synonyms: TP53 (acK382)
Pathways Signalisation p53, Signalisation MAPK, Signalisation PI3K-Akt, Apoptose, AMPK Signaling, Chromatin Binding, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Autophagy
Indications d'application Optimal working dilution should be determined by the investigator.
Volume d'échantillon 20 μL
Restrictions For Research Use only
Buffer Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.
Agent conservateur Sodium azide
Précaution d'utilisation This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Conseil sur la manipulation The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed.
Stock 4 °C
Stockage commentaire Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Produit citée dans: Nishino, Wang, Mochizuki-Kashio, Osawa, Nakauchi, Iwama: "Ex vivo expansion of human hematopoietic stem cells by garcinol, a potent inhibitor of histone acetyltransferase." dans: PLoS ONE, Vol. 6, Issue 9, pp. e24298, 2011 (PubMed).

Soliman, Riabowol: "After a decade of study-ING, a PHD for a versatile family of proteins." dans: Trends in biochemical sciences, Vol. 32, Issue 11, pp. 509-19, 2007 (PubMed).

Solomon, Pasupuleti, Xu, McDonagh, Curtis, DiStefano, Huber: "Inhibition of SIRT1 catalytic activity increases p53 acetylation but does not alter cell survival following DNA damage." dans: Molecular and cellular biology, Vol. 26, Issue 1, pp. 28-38, 2005 (PubMed).

Pedeux, Sengupta, Shen, Demidov, Saito, Onogi, Kumamoto, Wincovitch, Garfield, McMenamin, Nagashima, Grossman, Appella, Harris: "ING2 regulates the onset of replicative senescence by induction of p300-dependent p53 acetylation." dans: Molecular and cellular biology, Vol. 25, Issue 15, pp. 6639-48, 2005 (PubMed).

Hofmann, Möller, Sirma, Zentgraf, Taya, Dröge, Will, Schmitz: "Regulation of p53 activity by its interaction with homeodomain-interacting protein kinase-2." dans: Nature cell biology, Vol. 4, Issue 1, pp. 1-10, 2002 (PubMed).

Saito, Goodarzi, Higashimoto, Noda, Lees-Miller, Appella, Anderson: "ATM mediates phosphorylation at multiple p53 sites, including Ser(46), in response to ionizing radiation." dans: The Journal of biological chemistry, Vol. 277, Issue 15, pp. 12491-4, 2002 (PubMed).

Ito, Lai, Zhao, Saito, Hamilton, Appella, Yao: "p300/CBP-mediated p53 acetylation is commonly induced by p53-activating agents and inhibited by MDM2." dans: The EMBO journal, Vol. 20, Issue 6, pp. 1331-40, 2001 (PubMed).

Vaziri, Dessain, Ng Eaton, Imai, Frye, Pandita, Guarente, Weinberg: "hSIR2(SIRT1) functions as an NAD-dependent p53 deacetylase." dans: Cell, Vol. 107, Issue 2, pp. 149-59, 2001 (PubMed).

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