SHP1 anticorps

N° du produit ABIN271911

Cet anticorps anti-SHP1 Polyclonal Lapin (ABIN271911) détecte spécifiquement SHP1 dans WB et IHC (p). L’anticorps est réactif avec des échantillons de Humain, Souris et Rat.

Aperçu rapide pour SHP1 anticorps (ABIN271911)

Antigène: SHP1 (PTPN6) (Protein-tyrosine Phosphatase 1C (PTPN6))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp SHP1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-SHP1 anticorps

Specificité: This antibody detects endogenous levels of PTPN6 / SHP-1 protein (region surrounding Lys530).

Réactivité croisée (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Pureté: > 95 % pure by SDS-PAGE

Information d'application

Indications d'application: ELISA: 1: 20000approx. 1: 40000. WB: 1: 500approx. 1: 1000. IHC: 1: 50approx. 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS), approx. pH 7.2., 15 mM Sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: DO NOT FREEZE!

Stock: 4 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C.

Détails sur SHP1

Antigène: SHP1 (PTPN6) (Protein-tyrosine Phosphatase 1C (PTPN6))

Autre désignation: PTPN6

Sujet: SHP (also designated short heterodimer partner and small heterodimer partner) is an orphan nuclear receptor containing the dimerization and ligandbinding domains found in other nuclear receptors but lacking the conserved DNA binding domain. SHP is specifically expressed in liver and other tissues including fetal liver and adrenal gland, adult spleen and small intestine. In addition, SHP is highy expressed in the murine macrophage cell line RAW 264.7 but suppressed by oxLDL and 13-HODE, which is a ligand for PPARγ. SHP interacts with nuclear receptors including thyroid receptor, retinoic acid receptors (RAR and RXR) and estrogen receptors (ERa and ERb). SHP functions as a negative regulator of these receptors by at least three mechanisms: inhibition of DNA binding via dimerization, direct antagonism of coactivator function via competition and possibly transrepression via recruitment of putative corepressors. In oxLDL-treated, resting macrophage cells, SHP acts as a transcription coactivator of NF-κB, suggesting that SHP is a modulatory component in the regulation of the transcriptional activities of NF-κB. Lastly, negative feedback regulation of a hepatic bile acid transporter NTCP is controlled by bile acid-activated FXR via induction of SHP to protect the hepatocyte from bile acid-mediated damage in cholestatic conditions.Synonyms: HCP, Hematopoietic cell protein-tyrosine phosphatase, PTP-1C, PTP1C, PTPN-6, Protein-tyrosine phosphatase 1C, Protein-tyrosine phosphatase SHP-1, SH-PTP1, Tyrosine-protein phosphatase non-receptor type 6

Poids moléculaire: approx. 68 kDa

ID gène: 5777

NCBI Accession: NP_002822

UniProt: P29350

Pathways: Signalistation JAK/STAT, TCR Signaling, Signalisation TLR, Nuclear Receptor Transcription Pathway, Positive Regulation of Peptide Hormone Secretion, Steroid Hormone Mediated Signaling Pathway, Response to Growth Hormone Stimulus, Regulation of Leukocyte Mediated Immunity, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, BCR Signaling