MTOR anticorps

N° du produit ABIN272127

Cité dans 5 publications. Cet anticorps anti-MTOR Polyclonal Lapin (ABIN272127) détecte spécifiquement MTOR dans WB et IHC (p). L’anticorps est réactif avec des échantillons de Humain, Souris et Rat.

Aperçu rapide pour MTOR anticorps (ABIN272127)

Antigène: MTOR (mTOR) (Mechanistic Target of Rapamycin (serine/threonine Kinase) (mTOR))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp MTOR est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-MTOR anticorps

Specificité: This antibody detects endogenous levels of mTOR protein. (region surrounding Ser2442)

Réactivité croisée (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: Affinity Chromatography using epitope-specific immunogen

Information d'application

Indications d'application: ELISA: 1: 5000approx. 1: 10000. WB: 1: 500approx. 1: 1000. IHC: 1: 50approx. 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH ~7.2, 0.05 % Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Références pour anticorps anti-MTOR (ABIN272127)

Izumi, Nakamura, Mano, Onoda: "Well-differentiated pancreatic ductal adenocarcinomas measuring ≤ 1 cm exhibit early features of tumor progression: a report of five lesions and a comparative study with advanced lesions." dans: Surgery today, Vol. 44, Issue 11, pp. 2058-64, (2015) (PubMed).

Zhang, Kong, Chen, Zhang, Lian, Zhu, Lu, Zheng: "Peroxisome proliferator-activated receptor-? interrupts angiogenic signal transduction by transrepression of platelet-derived growth factor-? receptor in hepatic stellate cells." dans: Journal of cell science, Vol. 127, Issue Pt 2, pp. 305-14, (2014) (PubMed).

Li, Yan, Zhang, Jiang, Sun, Hu, Sun, Xu: "miR-145 inhibits isoproterenol-induced cardiomyocyte hypertrophy by targeting the expression and localization of GATA6." dans: FEBS letters, Vol. 587, Issue 12, pp. 1754-61, (2013) (PubMed).

Sun, Zou, Hu, Qin, Song, Lu, Guo: "Wogonoside induces autophagy in MDA-MB-231 cells by regulating MAPK-mTOR pathway." dans: Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association, Vol. 51, pp. 53-60, (2012) (PubMed).

Li, Xia, Huang, Chen, Su, Li, Wang, Ding, Shao: "A strategy to rapidly identify the functional targets of microRNAs by combining bioinformatics and mRNA cytoplasmic/nucleic ratios in culture cells." dans: FEBS letters, Vol. 584, Issue 14, pp. 3198-202, (2010) (PubMed).

Détails sur MTOR

Antigène: MTOR (mTOR) (Mechanistic Target of Rapamycin (serine/threonine Kinase) (mTOR))

Autre désignation: mTOR / FRAP1

Sujet: MTOR, or FKBP12-rapamycin associated protein (FRAP), is one of a family of proteins involved in cell cycle progression, DNA recombination, and DNA damage detection. In rat, it is a 245-kD protein (symbolized RAFT1) with significant homology to the Saccharomyces cerevisiae protein TOR1 and has been shown to associate with the immunophilin FKBP12 in a rapamycin-dependent fashion. The FKBP12-rapamycin complex is known to inhibit progression through the G1 cell cycle stage by interfering with mitogenic signaling pathways involved in G1 progression in several cell types, as well as in yeast. The binding of FRAP to FKBP12-rapamycin correlated with the ability of these ligands to inhibit cell cycle progression.Synonyms: FK506-binding protein 12-rapamycin complex-associated protein 1, FKBP12-rapamycin complex-associated protein, FRAP, FRAP2, Mammalian target of rapamycin, RAPT1, Rapamycin target protein, Serine/threonine-protein kinase mTOR, TOR

Poids moléculaire: approx. 289 kDa

ID gène: 2475

NCBI Accession: NP_004949

UniProt: P42345

Pathways: Signalisation PI3K-Akt, Signalisation RTK, AMPK Signaling, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Actin Filament Polymerization, Regulation of Muscle Cell Differentiation, Regulation of Cell Size, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Autophagy, CXCR4-mediated Signaling Events, BCR Signaling, L'effet Warburg