PAG1 anticorps (N-Term)

N° du produit ABIN2745550

Détail du produit anti-PAG1 anticorps

Antigène: PAG1 (phosphoprotein Associated with Glycosphingolipid Microdomains 1 (PAG1))

Épitope: AA 412-424, N-Term

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp PAG1 est non-conjugé

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Specificité: Recognizes mouse, rat and human PAG/Cbp.

Réactivité croisée: Humain, Souris, Rat (Rattus)

Purification: Antigen affinity purified.

Immunogène: Synthetic peptide corresponding to aa 412-424 of the N-terminal rat PAG including an additional N-terminal Cys residue.

Information d'application

Indications d'application: Positive Control for Western Blot: Use 1.5x106 total splenocytes per lane.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: In PBS containing 0.02 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Short Term Storage: +4°C
Long Term Storage: -20°C
Stable for at least 1 year after receipt when stored at -20°C.

Date de péremption: 12 months

Détails sur PAG1

Antigène: PAG1 (phosphoprotein Associated with Glycosphingolipid Microdomains 1 (PAG1))

Autre désignation: PAG/Cbp (PAG1 Produits)

Synonymes: anticorps Cbp, anticorps F730007C19Rik, anticorps Pag, anticorps CBP, anticorps PAG, anticorps MGC80192, anticorps phosphoprotein associated with glycosphingolipid microdomains 1, anticorps phosphoprotein membrane anchor with glycosphingolipid microdomains 1, anticorps phosphoprotein membrane anchor with glycosphingolipid microdomains 1 L homeolog, anticorps Pag1, anticorps PAG1, anticorps pag1.L, anticorps pag1

Sujet: PAG is a ubiquitously expressed transmembrane adapter protein. Depending on its phosphorylation status, it appears as diffuse band(s) of 80-90 kDa by SDS-PAGE, with phosphorylation leading to a shift towards a higher apparent molecular mass. Tyrosine phosphorylated PAG recruits Csk to the membrane and negatively regulates Src family kinases via Csk-mediated phosphorylation. Upon T cell receptor engagement, PAG becomes dephosphorylated, thereby displacing Csk from the membrane and enabling the activation of the Src kinases Fyn and Lck. PAG negatively regulates TCR (T cell antigen receptor)-mediated signaling in T cells and FCER1 (high affinity immunoglobulin epsilon receptor)-mediated signaling in mast cells. It promotes CSK activation and recruitment to lipid rafts, which results in LCK inhibition. It inhibits immunological synapse formation by preventing dynamic arrangement of lipid raft proteins and may be involved in cell adhesion signaling.

UniProt: Q9JM80

Pathways: Signalisation p53, TCR Signaling, EGFR Signaling Pathway, CXCR4-mediated Signaling Events