HLA-DR anticorps (APC)

N° du produit ABIN2749148

Cet anticorps Souris Monoclonal détecte spécifiquement HLA-DR dans FACS. Il présente une réactivité avec des échantillons de Humain, Chien et Non-Human Primate. Il a été cité dans 5+ publications.

Aperçu rapide pour HLA-DR anticorps (APC) (ABIN2749148)

Antigène: HLA-DR

Reactivité: Humain, Chien, Non-Human Primate

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp HLA-DR est conjugé à/à la APC

Application: Flow Cytometry (FACS)

Clone: L243

Détail du produit anti-HLA-DR anticorps (APC)

Fonction: Anti-HLA-DR APC

Specificité: The mouse monoclonal antibody L243 recognizes specifically an extracellular epitope on HLA-DR molecules, both peptide-loaded and empty.

Réactivité croisée (Details): Human, Non-Human Primates, Canine (Dog)

Purification: Purified antibody is conjugated with activated allophycocyanin (APC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Immunogène: Human B lymphocytes

Isotype: IgG2a kappa

Information d'application

Indications d'application: Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μL reagent / 100 μL of whole blood or 10⁶ cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.

Restrictions: For Research Use only

Stockage

Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

Références pour anticorps anti-HLA-DR (APC) (ABIN2749148)

Ivanov, Beers, Walshe, Honeychurch, Alduaij, Cox, Potter, Murray, Chan, Klymenko, Erenpreisa, Glennie, Illidge, Cragg: "Monoclonal antibodies directed to CD20 and HLA-DR can elicit homotypic adhesion followed by lysosome-mediated cell death in human lymphoma and leukemia cells." dans: The Journal of clinical investigation, Vol. 119, Issue 8, pp. 2143-59, (2009) (PubMed).

De Gassart, Camosseto, Thibodeau, Ceppi, Catalan, Pierre, Gatti: "MHC class II stabilization at the surface of human dendritic cells is the result of maturation-dependent MARCH I down-regulation." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 105, Issue 9, pp. 3491-6, (2008) (PubMed).

Swiatek-de Lange, Rist, Stahl, Weith, Lenter: "Comment on "MHC class II expression identifies functionally distinct human regulatory T cells"." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 180, Issue 6, pp. 3625; author reply 3626, (2008) (PubMed).

Bouillon, El Fakhry, Girouard, Khalil, Thibodeau, Mourad: "Lipid raft-dependent and -independent signaling through HLA-DR molecules." dans: The Journal of biological chemistry, Vol. 278, Issue 9, pp. 7099-107, (2003) (PubMed).

Kalka-Moll, Tzianabos, Bryant, Niemeyer, Ploegh, Kasper: "Zwitterionic polysaccharides stimulate T cells by MHC class II-dependent interactions." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 169, Issue 11, pp. 6149-53, (2002) (PubMed).

Détails sur HLA-DR

Antigène: HLA-DR

Autre désignation: HLA-DR

Sujet: HLA-DR, a member of MHC class II glycoproteins, that bind intracellularly processed peptides and present them to the Th cells, is composed of 36 kDa alpha chain and 27 kDa beta chain, both anchored in the plasma membrane. Together with other MHC II molecules HLA-DR plays a central role in the immune system.

Pathways: Human Leukocyte Antigen (HLA) in Adaptive Immune Response