Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF)
Specificité
This antibody is specific for bromodeoxyuridine and does not cross react with thymidine.
Réactivité croisée
Hamster, Humain, Souris, Lapin, Rat (Rattus)
Réactivité croisée (Details)
This antibody reacts with the 85 kDa ß-chain of the human LRP/alpha MR which 2is expressed in a restricted spectrum of cell types. Cross-reacts with rabbit LDL receptor-related protein (LRP) or mouse LRPAntigen detected in Human, rabbit, rat, hamster and mouse.
Immunogène
LRP antibody (85 kDa) was raised in mouse using human LRP/a2 MR as the immunogen.
IHC: 1:20 Optimal conditions should be determined by the investigator.
Restrictions
For Research Use only
Format
Lyophilized
Reconstitution
Reconstitute in dist. water (final solution contains 0.5 % BSA in PBS buffer, pH 7.4)
Concentration
Lot specific
Buffer
Lyophilized.
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Conseil sur la manipulation
Avoid repeated freeze/thaw cycles. Dilute only prior to immediate use.
Stock
4 °C/-20 °C
Stockage commentaire
Store at 4 °C until reconstitution. Following reconstitution aliquot and freeze at -20 °C for long term storage.
Bornachea, Benitez-Amaro, Vea, Nasarre, de Gonzalo-Calvo, Escola-Gil, Cedo, Iborra, Martínez-Martínez, Juarez, Camara, Espinet, Borrell-Pages, Badimon, Castell, Llorente-Cortés: "Immunization with the Gly1127-Cys1140 amino acid sequence of the LRP1 receptor reduces atherosclerosis in rabbits. Molecular, immunohistochemical and nuclear imaging studies." dans: Theranostics, Vol. 10, Issue 7, pp. 3263-3280, (2020) (PubMed).
de Gonzalo-Calvo, López-Vilaró, Nasarre, Perez-Olabarria, Vázquez, Escuin, Badimon, Barnadas, Lerma, Llorente-Cortés: "Intratumor cholesteryl ester accumulation is associated with human breast cancer proliferation and aggressive potential: a molecular and clinicopathological study." dans: BMC cancer, Vol. 15, pp. 460, (2016) (PubMed).