ARPC2 anticorps
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- Antigène Voir toutes ARPC2 Anticorps
- ARPC2 (Actin Related Protein 2/3 Complex, Subunit 2, 34kDa (ARPC2))
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Reactivité
- Humain
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Hôte
- Lapin
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Clonalité
- Polyclonal
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Conjugué
- Cet anticorp ARPC2 est non-conjugé
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Application
- Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Réactivité croisée
- Humain, Souris, Rat, Poisson zèbre (Danio rerio)
- Attributs du produit
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Rabbit Polyclonal antibody to ARPC2 (actin related protein 2/3 complex, subunit 2, 34 kDa)
ARPC2 antibody - Purification
- Purified by antigen-affinity chromatography.
- Immunogène
- Recombinant protein encompassing a sequence within the center region of human ARPC2. The exact sequence is proprietary.
- Isotype
- IgG
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- Indications d'application
- WB: 1:500-1:5000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.
- Commentaires
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Positive Control: rat kidney , mouse kidney
- Restrictions
- For Research Use only
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- by
- Akoya Biosciences
- No.
- #104342
- Date
- 20.04.2021
- Antigène
- ARPC2
- Numéro du lot
- 39911
- Application validée
- Multiplex Immunohistochemistry
- Contrôle positif
FFPE normal human cortex
- Contrôle négative
Unlabeled control (FFPE normal human cortex)
- Conclusion
Passed. The anti-ARPC2 antibody ABIN2855249 produced staining in neuronal processes throughout the human cortex.
- Anticorps primaire
- ABIN2855249
- Anticorps secondaire
- Full Protocol
- Protocol details are described in the Akoya Biosciences CODEX® User Manual (see https://www.akoyabio.com/wp-content/uploads/2021/01/CODEX-User-Manual.pdf).
- Tissue preparation as outlined in the Akoya Biosciences CODEX® User Manual fresh-frozen tissue protocol.
- Conjugation of the anti-ARPC2 antibody ABIN2855249 to an oligo barcode used to bind oligo-conjugated fluorophore AF488.
- Notes
No signal was detected in unlabeled specimens.
Specific staining of ARPC2 was observed with human FFPE cortical tissue sections with tris-EDTA antigen retrieval.
Anti-ARPC antibody ABIN2855249 was weakly compatible with staining of fresh frozen mouse tissue
- Optimal staining and signal to noise ratios were obtained if tissue was pre-treated for autofluorescence removal (see https://www.akoyabio.com/wp-content/uploads/2020/07/Customer-Demonstrated-Protocol-Autofluorescence-Quenching-Mar2020.pdf).
Validation #104342 (Multiplex Immunohistochemistry)Validation ImagesFFPE normal human cortex tissue section labeled with anti-ARPC2 antibody ABIN2855249 (cyan; conjugated to fluorophore AF488) after EDTA antigen retrieval. MAP2, neurofilament, and GFAP were labeled with anti-MAP2 antibody ABIN125739 (green; conjugated to fluorophore ATTO 550), anti-neurofilament antibody (red; conjugated to fluorophore AF488), and anti-GFAP antibody (purple; conjugated to fluorophore AF647) respectively.Protocole -
- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- 1XPBS ( pH 7), 20 % Glycerol, 0.025 % ProClin 300
- Agent conservateur
- ProClin
- Précaution d'utilisation
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Stock
- 4 °C,-20 °C
- Stockage commentaire
- Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
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- Antigène
- ARPC2 (Actin Related Protein 2/3 Complex, Subunit 2, 34kDa (ARPC2))
- Autre désignation
- actin related protein 2/3 complex subunit 2 (ARPC2 Produits)
- Synonymes
- anticorps arc34, anticorps p34-arc, anticorps pnas-139, anticorps pro2446, anticorps Arpc2, anticorps Arc-p34, anticorps 2210023N03Rik, anticorps 34kDa, anticorps p34-Arc, anticorps fk84a07, anticorps wu:fa22f07, anticorps wu:fk84a07, anticorps zgc:77769, anticorps ARC34, anticorps PNAS-139, anticorps actin related protein 2/3 complex subunit 2, anticorps actin related protein 2/3 complex, subunit 2, 34kDa, anticorps actin related protein 2/3 complex, subunit 2, anticorps actin related protein 2/3 complex subunit 2 S homeolog, anticorps ARPC2, anticorps arpc2, anticorps Arpc2, anticorps arpc2.S
- Sujet
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This gene encodes one of seven subunits of the human Arp2/3 protein complex. The Arp2/3 protein complex has been implicated in the control of actin polymerization in cells and has been conserved through evolution. The exact role of the protein encoded by this gene, the p34 subunit, has yet to be determined. Two alternatively spliced variants have been characterized to date. Additional alternatively spliced variants have been described but their full length nature has not been determined.
Cellular Localization: Cytoplasm , cytoskeleton , Cell projection - Poids moléculaire
- 34 kDa
- ID gène
- 10109
- UniProt
- O15144
- Pathways
- Signalisation RTK, Regulation of Actin Filament Polymerization
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