ATP6V1H anticorps (Center)

N° du produit ABIN2856776

Détail du produit anti-ATP6V1H anticorps

Antigène: ATP6V1H (ATPase, H+ Transporting, Lysosomal 50/57kDa, V1 Subunit H (ATP6V1H))

Épitope: Center

Reactivité: Humain, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp ATP6V1H est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Immunocytochemistry (ICC)

Réactivité croisée: Poulet, Porc, Xenopus laevis, Poisson zèbre (Danio rerio), Boeuf (Vache), Xenopus tropicalis

Réactivité croisée (Details): Chicken (96 %), Pig (99 %), Xenopus laevis (94 %), Zebrafish (91 %), Bovine (98 %), Xenopus tropicalis (94 %)

Attributs du produit: Rabbit Polyclonal antibody to ATP6V1H (ATPase, H+ transporting, lysosomal 50/57 kDa, V1 subunit H)
ATP6V1H antibody [N3C3]

Purification: Purified by antigen-affinity chromatography.

Immunogène: Recombinant protein encompassing a sequence within the center region of human ATP6V1H. The exact sequence is proprietary.

Isotype: IgG

Information d'application

Indications d'application: Suggested dilution Reference ICC/IF Assay-dependent dilution IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IFAssay-dependent dilution IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Western blot1:500-1:3000*

Commentaires:

Positive Control: 293T , A431 , H1299 , HeLa , Raji , mouse brain

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.56 mg/mL

Buffer: 0.1M Tris, 0.1M Glycine, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.

Agent conservateur: Thimerosal (Merthiolate)

Précaution d'utilisation: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Détails sur ATP6V1H

Antigène: ATP6V1H (ATPase, H+ Transporting, Lysosomal 50/57kDa, V1 Subunit H (ATP6V1H))

Autre désignation: ATP6V1H (ATP6V1H Produits)

Synonymes: anticorps MSTP042, anticorps NBP1, anticorps SFD, anticorps SFDalpha, anticorps SFDbeta, anticorps VMA13, anticorps V-ATPASE, anticorps 0710001F19Rik, anticorps AU022349, anticorps CGI-11, anticorps chunp6890, anticorps ATPase H+ transporting V1 subunit H, anticorps ATPase, H+ transporting, lysosomal V1 subunit H, anticorps ATP6V1H, anticorps Atp6v1h, anticorps atp6v1h

Sujet: This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c", and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This gene encodes the regulatory H subunit of the V1 domain which is required for catalysis of ATP but not the assembly of V-ATPase. Three alternatively spliced transcript variants encode two isoforms of the H subunit.

Poids moléculaire: 56 kDa

ID gène: 51606

Pathways: Transition Metal Ion Homeostasis, Proton Transport