His Tag anticorps
-
- Antigène Voir toutes His Tag Anticorps
- His Tag
- Reactivité
- Veuillez nous consulter SVP
-
Hôte
- Lapin
-
Clonalité
- Polyclonal
-
Conjugué
- Cet anticorp His Tag est non-conjugé
-
Application
- Western Blotting (WB), ELISA
- Séquence
- HHHHHH
- Specificité
- The 6X His motif and is useful in determining its presence in various assays. This polyclonal anti-6X His tag antibody detects over-expressed proteins containing the 6X His epitope tag. To date, this antibody has reacted with all His tagged proteins so far tested. In western blotting of bacterial extracts, the antibody does not cross-react with endogenous proteins. The antibody recognizes the His-tag (His-His-His-His-His-His) fused to either the amino- or carboxy- termini of targeted proteins in transfected or transformed cells
- Purification
- Affinity purified
- Immunogène
- 6X His epitope tag peptide H-H-H-H-H-H conjugated to KLH using maleimide.
- Top Product
- Discover our top product His Tag Anticorps primaire
-
-
- Indications d'application
-
Approved: ELISA (1:10000 - 1:40000), WB (1:1000 - 1:3000)
Usage: This antibody has been tested by ELISA and western blotting against both the immunizing peptide and His-containing recombinant proteins. Although not tested, this antibody is likely functional for immunoprecipitation and immunocytochemistry. - Restrictions
- For Research Use only
-
- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- 0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01 % sodium azide.
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Conseil sur la manipulation
- Aliquot to Avoid freeze/thaw cycles. Store undiluted.
- Stock
- -20 °C
- Stockage commentaire
- Store at -20°C. Aliquot to avoid freeze-thaw cycles. Store undiluted.
-
- Antigène
- His Tag
- Abstract
- His Tag Produits
- Classe de substances
- Tag
-