PRKACA anticorps

N° du produit ABIN302080

Cet anticorps Souris Monoclonal détecte spécifiquement PRKACA dans WB. Il présente une réactivité avec des échantillons de Humain.

Aperçu rapide pour PRKACA anticorps (ABIN302080)

Antigène: PRKACA (Protein Kinase A, alpha (PRKACA))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp PRKACA est non-conjugé

Application: Western Blotting (WB)

Clone: 6D2-1

Détail du produit anti-PRKACA anticorps

Fonction: Anti-PKAc Purified

Réactivité croisée (Details): Human, Other not determined

Attributs du produit: The mouse monoclonal antibody 6D2.1 strongly reacts with human proteinkinase A (an intracellular antigen) catalytic (PKAc) alpha subunit, and weakly with PKAc gamma subunit (both around 40 kDa). The recognized epitope of PKAc alpha is identical between man, sheep, pig, ox and dog.

Purification: Purified by protein-A affinity chromatography.

Pureté: > 95 % (by SDS-PAGE)

Immunogène: Peptide corresponding to amino acids ESPAQNTAHLDQFERIK of human proteinkinase A c alpha (PKAc alpha).

Isotype: IgG1

Information d'application

Indications d'application: Western blotting: Recommended dilution: 1 μg/mL, positive control: HeLa human cervix carcinoma cell line. To detect PKAc gamma, use a more concentrated lysate from a tissue expressing this subunit (testis).

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Do not freeze

Stock: 4 °C

Stockage commentaire: Store at 2-8°C. Do not freeze.

Détails sur PRKACA

Antigène: PRKACA (Protein Kinase A, alpha (PRKACA))

Autre désignation: PKAc

Sujet: Protein kinase cAMP-activated catalytic subunit al,Protein kinase A (PKA, cAMP-dependent protein kinase) is a key element of a ubiquitous signaling pathway important in the cell cycle, cellular communication, memory formation and behavior. PKA is composed of two catalytic (PKAc, proteinkinase A catalytic subunit) and two regulatory subunits (PKAr). Upon binding cAMP, the complex dissociates to PKAr dimer and two activated PKAc ser/thr protein kinase catalytic monomers. The released PKAc can translocate into the nucleus and exert a regulatory role in the activation of multiple nuclear hormone receptors. However, PKAc-mediated activation of tonicity-dependent gene expression is cAMP independent. Humans express three types of PKAc subunit –, PKAc alpha is present in most human tissues, PKAc beta and gamma are tissue-specific, the later is found in testes.,PKACA, PPNAD4, PRKACA, Proteinkinase Ac

ID gène: 5566

UniProt: P17612

Pathways: Signalisation NF-kappaB, Signalisation Hedgehog, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Thyroid Hormone Synthesis, Carbohydrate Homeostasis, Myometrial Relaxation and Contraction, M Phase, G-protein mediated Events, Signaling Events mediated by VEGFR1 and VEGFR2, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, VEGFR1 Specific Signals, Lipid Metabolism, SARS-CoV-2 Protein Interactome, Phosphorylation & l'infection par le SRAS-CoV-2