HRAS anticorps

N° du produit ABIN302083

Cet anticorps Souris Monoclonal détecte spécifiquement HRAS dans WB. Il présente une réactivité envers Humain.

Aperçu rapide pour HRAS anticorps (ABIN302083)

Antigène: HRAS (HRas proto-oncogene, GTPase (HRAS))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp HRAS est non-conjugé

Application: Western Blotting (WB)

Clone: H-RAS-03

Détail du produit anti-HRAS anticorps

Fonction: Anti-H-RAS Purified

Specificité: The mouse monoclonal antibody H-RAS-03 reacts with human H-Ras, an ubiquitously expressed 21 kDa intracellular protein. Although reactivity with other species has not been determined, it is probable as the epitope is highly conserved among animals.

Réactivité croisée (Details): Human, Other not determined

Purification: Purified by protein-A affinity chromatography.

Pureté: > 95 % (by SDS-PAGE)

Immunogène: Peptide corresponding to amino acids DIHQYREQIKRVKDSDDC of human H-Ras protein

Isotype: IgG1

Information d'application

Indications d'application: Western blotting: Recommended dilution: 1-2 μg/mL, reducing conditions.

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Do not freeze.

Stock: 4 °C

Stockage commentaire: Store at 2-8°C. Do not freeze.

Détails sur HRAS

Antigène: HRAS (HRas proto-oncogene, GTPase (HRAS))

Autre désignation: H-RAS

Sujet: HRas proto-oncogene,H-Ras is one of three ubiquitously isoforms of Ras GTPase that operate at the intracellular leaflet of the plasma membrane to regulate multiple signal transduction pathways, such as mitogen-activated protein kinase (MAPK) cascade. H-Ras is anchored to the plasma membrane by farnesyl and two palmityl residues. GTP loading decreases H-Ras affinity for lipid rafts and allows the protein to target to nonraft microdomains, the primary sites of H-Ras signaling. Sos protein and other guanine nucleotide-exchange factors catalyze dissociation of GDP from Ras. Besides its roles in the plasma membrane, active H-Ras also diffuses through the cytoplasm on nanoparticles termed rasosomes, which is dependent on Ras palmitoylation.,Ras-H, HRAS, p21Ras

ID gène: 3265

UniProt: P01112

Pathways: Signalisation p53, Signalisation MAPK, Signalisation RTK, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Hepatitis C, Autophagy, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, Regulation of long-term Neuronal Synaptic Plasticity, VEGF Signaling, BCR Signaling