GTPase NRas anticorps (Internal Region)

N° du produit ABIN302138

L’anticorps Chèvre Polyclonal anti-GTPase NRas a été validé pour WB et ELISA. Il convient pour détecter GTPase NRas dans des échantillons de Humain.

Aperçu rapide pour GTPase NRas anticorps (Internal Region) (ABIN302138)

Antigène: GTPase NRas (NRAS)

Reactivité: Humain

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp GTPase NRas est non-conjugé

Application: Western Blotting (WB), ELISA

Classe de qualité: Verified

Détail du produit anti-GTPase NRas anticorps

Épitope: Internal Region

Fonction: NRAS

Séquence: RMKKLNSSDD GTQ

Réactivité croisée: Humain, Souris, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogène: Peptide with sequence C-RMKKLNSSDDGTQ, from the internal region of the protein sequence according to NP_002515.1.

Isotype: IgG

Information d'application

Indications d'application:

Western Blot: Approx. 21-22 kDa band observed in lysates of cell line MCF7 (calculated MW of 21.2 kDa according to NP_002515.1). Recommended concentration: 0.3-1 μg/mL. Primary incubation1 hour at room temperature.

Peptide ELISA: antibody detection limit dilution 1:64000.

Commentaires:

Immunofluorescence: A customer obtained weak cytoplasmic staining in MCF7 cells at 10μg/ml.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Détails sur GTPase NRas

Antigène: GTPase NRas (NRAS)

Autre désignation: NRAS

Sujet: NRAS , neuroblastoma RAS viral (v-ras) oncogene homolog , N-ras , NRAS1, N-ras protein part 4 , v-ras neuroblastoma RAS viral oncogene homolog

ID gène: 4893, 18176, 24605

NCBI Accession: NP_002515

Pathways: Signalisation p53, Signalisation MAPK, Signalisation RTK, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Hepatitis C, Regulation of long-term Neuronal Synaptic Plasticity, VEGF Signaling