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Histone 3 (H3) (H3K9me) anticorps Primary Antibody

H3 Reactivité: Humain, Souris, Rat ChIP, IF, IP, IHC, WB Hôte: Lapin Polyclonal
Pubmed (4)
N° du produit ABIN3023260
Plus shipping costs $45.00
local_shipping Destination: Etats-Unis
Envoi sous 13 à 14 jours ouvrables
  • Antigène
    Humain, Souris, Rat
    Chromatin Immunoprecipitation (ChIP), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
    Affinity purification
    A synthetic methylated peptide corresponding to residues surrounding K9 of human histone H3
  • Indications d'application
    Recommended dilutions: WB: 1:500-1:2000, IHC: 1:50-1:200, IF: 1:50-1:200, IP: 1:50-1:200, ChIP: 1:50-1:200
    For Research Use only
  • Format
    PBS with 0.02 % sodium azide, 50 % glycerol, pH 7.3
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Conseil sur la manipulation
    Avoid freeze / thaw cycles
    -20 °C/-80 °C
  • Zuo, Rong, Li, Lv, Lan, Tong: "The histone methyltransferase SETD2 is required for expression of acrosin-binding protein 1 and protamines and essential for spermiogenesis in mice." dans: The Journal of biological chemistry, Vol. 293, Issue 24, pp. 9188-9197, 2019 (PubMed). Méthode utilisée: Western Blotting (WB)

    Vaughan, Dickson, Whelihan, Johnstone, Cornett, Cheek, Ausherman, Cowles, Sun, Rothbart: "Chromatin structure and its chemical modifications regulate the ubiquitin ligase substrate selectivity of UHRF1." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 115, Issue 35, pp. 8775-8780, 2018 (PubMed). Méthode utilisée: Western Blotting (WB)

    Cao, Liu, Yue, Liu, Pei, Gu, Wang, Jia: "Iron chelation inhibits cancer cell growth and modulates global histone methylation status in colorectal cancer." dans: Biometals : an international journal on the role of metal ions in biology, biochemistry, and medicine, Vol. 31, Issue 5, pp. 797-805, 2018 (PubMed). Méthode utilisée: Western Blotting (WB)

    Zhao, Lei, Zhang, Zheng, Wang, Zhao, Li, Ye, Li, Wei, Wu: "Global histone modification profiling reveals the epigenomic dynamics during malignant transformation in a four-stage breast cancer model." dans: Clinical epigenetics, Vol. 8, pp. 34, 2016 (PubMed). Méthode utilisée: Western Blotting (WB)

  • Antigène
    Autre désignation
    Histone H3
    Modulation of chromatin structure plays an important role in the regulation of transcription in eukaryotes. The nucleosome, made up of DNA wound around eight core histone proteins (two each of H2A, H2B, H3, and H4), is the primary building block of chromatin. The amino-terminal tails of core histones undergo various post-translational modifications, including acetylation, phosphorylation, methylation, and ubiquitination. These modifications occur in response to various stimuli and have a direct effect on the accessibility of chromatin to transcription factors and, therefore, gene expression. In most species, histone H2B is primarily acetylated at Lys5, 12, 15, and 20. Histone H3 is primarily acetylated at Lys9, 14, 18, 23, 27, and 56. Acetylation of H3 at Lys9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms. Phosphorylation at Ser10, Ser28, and Thr11 of histone H3 is tightly correlated with chromosome condensation during both mitosis and meiosis. Phosphorylation at Thr3 of histone H3 is highly conserved among many species and is catalyzed by the kinase haspin. Immunostaining with phospho-specific antibodies in mammalian cells reveals mitotic phosphorylation at Thr3 of H3 in prophase and its dephosphorylation during anaphase.
    Synonyms: HIST1H3J , H3/j , H3FJ , Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h , Histone H3/I, Histone H3/j , Histone H3/k, Histone H3/l, HIST3H3
    ID gène
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