This mAb recognizes a protein of ~30 kDa, identified as CELA3B (Chymotrypsin like elastase family member 3B), or Elastase 3B. Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six genes which encode the structurally similar proteins: Elastase 1, 2, 2A, 2B, 3A, and 3B. Unlike other elastases, 3B has little elastolytic activity. Like most of the human elastases, 3B is secreted from the pancreas as a zymogen and, like other serine proteases such as trypsin, chymotrypsin and kallikrein, it has a digestive function in the intestine. Elastase 3B preferentially cleaves proteins after alanine residues. It may also function in the intestinal transport and metabolism of cholesterol. Elastases 3A/B have been referred to as Protease E and as Elastase 1, and excretion of this protein in fecal material is frequently used as a measure of pancreatic function in clinical assays.
Purification
Protein G affinity chromatography
Immunogène
A partial recombinant protein (aa 82-238) was used as the immunogen for the CELA3B antibody.
Optimal dilution of the CELA3B antibody should be determined by the researcher.\. ELISA : 2-4 μg/mL, order BSA free format for coating,Western blot: 1-2 μg/mL
Restrictions
For Research Use only
Concentration
0.2 mg/mL
Buffer
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Stock
4 °C,-20 °C
Stockage commentaire
Store the CELA3B antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
anticorps CBPP, anticorps E1, anticorps EL-1, anticorps ELA3B, anticorps 0910001F22Rik, anticorps 2310074F01Rik, anticorps AI504000, anticorps Ela3, anticorps Ela3b, anticorps ela3b, anticorps MGC82867, anticorps ELA1, anticorps Cela3b, anticorps CELA3B, anticorps chymotrypsin like elastase family member 3B, anticorps chymotrypsin-like elastase family, member 3B, anticorps elastase 3B, pancreatic, anticorps proproteinase E, anticorps chymotrypsin like elastase family member 3B L homeolog, anticorps chymotrypsin-like elastase family member 3B, anticorps CELA3B, anticorps Cela3b, anticorps ela3b, anticorps LOC478196, anticorps cela3b.L, anticorps LOC100717936, anticorps LOC100071692
Sujet
This mAb recognizes a protein of ~30 kDa, identified as CELA3B (Chymotrypsin like elastase family member 3B), or Elastase 3B. Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six genes which encode the structurally similar proteins: Elastase 1, 2, 2A, 2B, 3A, and 3B. Unlike other elastases, 3B has little elastolytic activity. Like most of the human elastases, 3B is secreted from the pancreas as a zymogen and, like other serine proteases such as trypsin, chymotrypsin and kallikrein, it has a digestive function in the intestine. Elastase 3B preferentially cleaves proteins after alanine residues. It may also function in the intestinal transport and metabolism of cholesterol. Elastases 3A/B have been referred to as Protease E and as Elastase 1, and excretion of this protein in fecal material is frequently used as a measure of pancreatic function in clinical assays.