CD31 anticorps
-
- Antigène Voir toutes CD31 (PECAM1) Anticorps
- CD31 (PECAM1) (Platelet/endothelial Cell Adhesion Molecule (PECAM1))
-
Reactivité
- Humain, Lapin, Cynomolgus
-
Hôte
- Souris
-
Clonalité
- Monoclonal
-
Conjugué
- Cet anticorp CD31 est non-conjugé
-
Application
- Flow Cytometry (FACS), Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Aucune reactivité croisée
- Rat (Rattus), Porc
- Purification
- Protein G affinity chromatography
- Immunogène
- A membrane preparation of a spleen from a patient with hairy cell leukemia was used as the immunogen for this CD31 antibody.
- Clone
- JC-70A
- Isotype
- IgG1 kappa
- Top Product
- Discover our top product PECAM1 Anticorps primaire
-
-
- Indications d'application
-
The optimal dilution of the CD31 antibody for each application should be determined by the researcher.
1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 0.5-1 μg/mL,Western blot: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 1-2 μg/mL for 30 minutes at RT (1),Prediluted format: incubate for 30 min at RT (2) - Restrictions
- For Research Use only
-
- Concentration
- 0.2 mg/mL
- Buffer
- 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Stock
- 4 °C,-20 °C
- Stockage commentaire
- Store the CD31 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
-
-
Adipose-Derived Stem Cells Cocultured with Chondrocytes Promote the Proliferation of Chondrocytes." dans: Stem cells international, Vol. 2017, pp. 1709582, (2017) (PubMed).
: "
-
Adipose-Derived Stem Cells Cocultured with Chondrocytes Promote the Proliferation of Chondrocytes." dans: Stem cells international, Vol. 2017, pp. 1709582, (2017) (PubMed).
-
- Antigène
- CD31 (PECAM1) (Platelet/endothelial Cell Adhesion Molecule (PECAM1))
- Autre désignation
- CD31 (PECAM1 Produits)
- Synonymes
- anticorps CD31, anticorps CD31/EndoCAM, anticorps GPIIA', anticorps PECA1, anticorps PECAM-1, anticorps endoCAM, anticorps C85791, anticorps Cd31, anticorps Pecam, anticorps platelet/endothelial cell adhesion molecule 1, anticorps platelet and endothelial cell adhesion molecule 1, anticorps pecam1, anticorps PECAM1, anticorps Pecam1
- Sujet
- CD31 (PECAM-1) is a transmembrane glycoprotein member of the immunoglobulin supergene family of adhesion molecules. CD31 is expressed by stem cells of the hematopoietic system and is primarily used to identify and concentrate these cells for experimental studies as well as for bone marrow transplantation. Anti-CD31 has shown to be highly specific and sensitive for vascular endothelial cells. Staining of nonvascular tumors (excluding hematopoietic neoplasms) is rare. CD31 mAb reacts with normal, benign, and malignant endothelial cells which make up blood vessel lining. The level of CD31 expression can help to determine the degree of tumor angiogenesis, and a high level of CD31 expression may imply a rapidly growing tumor and potentially a predictor of tumor recurrence.
- Pathways
- Regulation of Actin Filament Polymerization
-