c-MYC anticorps (C-Term)

N° du produit ABIN3025307

Cet anticorps Souris Monoclonal détecte spécifiquement c-MYC dans WB, IF, FACS et IHC (p). Il présente une réactivité avec des échantillons de Humain.

Aperçu rapide pour c-MYC anticorps (C-Term) (ABIN3025307)

Antigène: c-MYC (MYC) (Myc Proto-Oncogene protein (MYC))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp c-MYC est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: SPM237

Détail du produit anti-c-MYC anticorps

Épitope: C-Term

Aucune reactivité croisée: Souris, Rat (Rattus), Poulet

Purification: Protein G affinity chromatography

Immunogène: Amino acids AEEQKLISEEDLLRKRREQLKHKLEQLRNSCA from the C-terminus was used as the immunogen for the Myc antibody.

Isotype: IgG1 kappa

Information d'application

Indications d'application: Optimal dilution of the Myc antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Tris with 1  mM EDTA,  pH 9.0, for 10-20 min followed by cooling at RT for 20 min
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Western blot: 1-2 μg/mL, Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 1-2 μg/mL,Immunohistochemistry (FFPE): 1-2 μg/mL for 30 min at RT (1),Prediluted format : incubate for 30 min at RT (2)

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Agent conservateur: Azide free

Stock: 4 °C,-20 °C

Stockage commentaire: Store the Myc antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Détails sur c-MYC

Antigène: c-MYC (MYC) (Myc Proto-Oncogene protein (MYC))

Autre désignation: Myc

Sujet: Myc, or c-Myc, is involved in the control of cell proliferation and differentiation and is amplified and/or overexpressed in a variety of tumors. Over-expression of c-Myc protein occurs frequently in luminal cells of prostate intraepithelial neoplasia as well as in most primary carcinomas and metastatic disease.

Pathways: Signalisation p53, Cycle Cellulaire, Sensory Perception of Sound, Transition Metal Ion Homeostasis, Mitotic G1-G1/S Phases, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Positive Regulation of Response to DNA Damage Stimulus, L'effet Warburg