p53 anticorps (N-Term)
Aperçu rapide pour p53 anticorps (N-Term) (ABIN3025364)
Antigène
Voir toutes p53 (TP53) AnticorpsReactivité
Hôte
Clonalité
Conjugué
Application
Classe de qualité
Clone
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Épitope
- AA 20-25, N-Term
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Fonction
- Anti-p53 Antibody (azide and preservative free)
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Aucune reactivité croisée
- Souris, Rat (Rattus)
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Purification
- Protein G affinity chromatography
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Immunogène
- Recombinant human wild type p53 protein was used as the immunogen for this anti-p53 antibody. Its epitope maps within the N-terminus (approx. amino acids 20-25).
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Isotype
- IgG2a, kappa
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Indications d'application
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The optimal dilution of the anti-p53 antibody for each application should be determined by the researcher.
1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10 mM Tris with 1 mM EDTA for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. -
Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 1 mg/mL
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Buffer
- 1 mg/mL in 1X PBS, BSA free, sodium azide free
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Agent conservateur
- Azide free
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Stock
- 4 °C,-20 °C
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Stockage commentaire
- Store the anti-p53 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
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- p53 (TP53) (Tumor Protein P53 (TP53))
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Autre désignation
- p53
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Sujet
- P53 is a tumor suppressor gene expressed in a wide variety of tissue types and is involved in regulating cell growth, replication, and apoptosis.
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UniProt
- P04637
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Pathways
- Signalisation p53, Signalisation MAPK, Signalisation PI3K-Akt, Apoptose, AMPK Signaling, Chromatin Binding, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Autophagy, L'effet Warburg
Antigène
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