Insulin anticorps

N° du produit ABIN3025691

Cet anticorps Souris Monoclonal détecte spécifiquement Insulin dans IF et IHC (p). Il présente une réactivité envers Humain, Porc, Souris, Boeuf (Vache) et Lapin.

Aperçu rapide pour Insulin anticorps (ABIN3025691)

Antigène: Insulin (INS)

Reactivité: Humain, Porc, Souris, Boeuf (Vache), Lapin

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Insulin est non-conjugé

Application: Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: IRDN-805

Détail du produit anti-Insulin anticorps

Aucune reactivité croisée: Rat (Rattus)

Purification: Protein G affinity chromatography

Immunogène: Recombinant INS protein was used as the immunogen for the Insulin antibody.

Isotype: IgG1 kappa

Information d'application

Indications d'application: Optimal dilution of the Insulin antibody should be determined by the researcher.

1. No special pretreatment is required for staining of formalin/paraffin tissues.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 1-2 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1),Prediluted format : incubate for 30 min at RT (2)

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Agent conservateur: Azide free

Stock: 4 °C,-20 °C

Stockage commentaire: Store the Insulin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Détails sur Insulin

Antigène: Insulin (INS)

Autre désignation: Insulin

Sujet: Recognizes a polypeptide which is identified as insulin, a 51-amino acid polypeptide composed of A and B chains connected through the C-peptide. Proinsulin, which has very little biological activity, is cleaved by proteases within its cell of origin into the insulin molecule and the C-terminal basic residue. Insulin enhances membrane transport of glucose, amino acids, and certain ions. It also promotes glycogen storage, formation of triglycerides, and synthesis of proteins and nucleic acids. Deficiency of insulin results in diabetes mellitus. The main storage site for insulin is the pancreatic islets. Antibodies to insulin are important as beta-cell and insulinoma marker.

Pathways: Signalisation NF-kappaB, Signalisation RTK, Positive Regulation of Peptide Hormone Secretion, Peptide Hormone Metabolism, Hormone Activity, Carbohydrate Homeostasis, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Autophagy, Negative Regulation of intrinsic apoptotic Signaling, Brown Fat Cell Differentiation, Positive Regulation of fat Cell Differentiation