Insulin anticorps (AA 1-84)

N° du produit ABIN3026328

L’anticorps Souris Monoclonal anti-Insulin a été validé pour IF et IHC (p). Il convient pour détecter Insulin dans des échantillons de Humain, Porc, Boeuf (Vache), Rat et Lapin.

Aperçu rapide pour Insulin anticorps (AA 1-84) (ABIN3026328)

Antigène: Insulin (INS)

Reactivité: Humain, Porc, Boeuf (Vache), Rat, Lapin

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Insulin est non-conjugé

Application: Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: E2-E3-2D11-H5 or INS04-INS05

Détail du produit anti-Insulin anticorps

Épitope: AA 1-84

Attributs du produit: This antibody cocktail recognizes a polypeptide which is identified as insulin, a 51-amino acid polypeptide composed of A and B chains connected through the C-peptide. Proinsulin, which has very little biological activity, is cleaved by proteases within its cell of origin into the insulin molecule and the C-terminal basic residue. It enhances membrane transport of glucose, amino acids, and certain ions. It also promotes glycogen storage, formation of triglycerides, and synthesis of proteins and nucleic acids. Deficiency of insulin results in diabetes mellitus. The main storage site for insulin is the pancreatic islets. Specific antibodies can be used as beta-cell and insulinoma markers.

Purification: Protein G affinity chromatography

Immunogène: Full length purified pig Insulin (aa 1-84), clones E2-E3 and 2D11-H5, was used as the immunogen for this antibody cocktail.

Isotype: IgG1 kappa

Information d'application

Indications d'application: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.

1. No special pretreatment is required for staining of formalin/paraffin tissues.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. FACS: 0.5-1 μg/million cells,IF: 1-2 μg/mL,IHC (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1),Prediluted format : incubate for 30 min at RT (2)

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Agent conservateur: Azide free

Stock: 4 °C,-20 °C

Stockage commentaire: Store the Insulin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Détails sur Insulin

Antigène: Insulin (INS)

Autre désignation: Insulin

Sujet: This antibody cocktail recognizes a polypeptide which is identified as insulin, a 51-amino acid polypeptide composed of A and B chains connected through the C-peptide. Proinsulin, which has very little biological activity, is cleaved by proteases within its cell of origin into the insulin molecule and the C-terminal basic residue. It enhances membrane transport of glucose, amino acids, and certain ions. It also promotes glycogen storage, formation of triglycerides, and synthesis of proteins and nucleic acids. Deficiency of insulin results in diabetes mellitus. The main storage site for insulin is the pancreatic islets. Specific antibodies can be used as beta-cell and insulinoma markers.

ID gène: 3630

Pathways: Signalisation NF-kappaB, Signalisation RTK, Positive Regulation of Peptide Hormone Secretion, Peptide Hormone Metabolism, Hormone Activity, Carbohydrate Homeostasis, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Autophagy, Negative Regulation of intrinsic apoptotic Signaling, Brown Fat Cell Differentiation, Positive Regulation of fat Cell Differentiation