Prostate Specific Antigen anticorps

N° du produit ABIN3026676

Détail du produit anti-Prostate Specific Antigen anticorps

Antigène: Prostate Specific Antigen (PSA)

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Prostate Specific Antigen est non-conjugé

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Flow Cytometry (FACS)

Attributs du produit: This antibody recognizes a single protein of 33-34 kDa, identified as the Prostate specific antigen. This PSA antibody is highly specific and stains prostatic secretory and ductal epithelium in both normal and neoplastic tissues. Prostate specific antigen is a chymotrypsin-like serine protease (kallikrein family) exclusively produced by the prostate epithelium, and abundant in seminal fluid. PSA can be detected in the sera of patients with prostatic carcinoma. It is predominantly complexed to a liver-derived serine protease inhibitor, alpha-1-antichymotrypsin (ACT). A higher proportion of serum PSA is complexed to ACT in prostate cancer than in benign hyperplasia.

Purification: Protein G affinity chromatography

Immunogène: Prostate Specific Antigen from human sperm plasma was used as the antigen for this PSA antibody.

Clone: A67-B-E3

Isotype: IgG1 kappa

Information d'application

Indications d'application: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the PSA antibody to be titered up or down for optimal performance.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 1  mM EDTA Buffer,  pH 7.5-8.5, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. FACS: 0.5-1 μg/million cells,IF: 0.5-1 μg/mL,IHC (FFPE): 0.5-1.0 μg/mL for 30 minutes at RT (1),Prediluted format : incubate for 30 min at RT (2)

Restrictions: For Research Use only

Stockage

Concentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store the PSA antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Détails sur Prostate Specific Antigen

Antigène: Prostate Specific Antigen (PSA)

Autre désignation: PSA Prostate Specific Antigen (PSA Produits)

Synonymes: anticorps APS, anticorps KLK2A1, anticorps PSA, anticorps hK3, anticorps KLK3, anticorps Klk1c10l2, anticorps RSGK-50, anticorps RSKG-50, anticorps rGK-3, anticorps Kal-3, anticorps Kal3, anticorps Klk3, anticorps AAP-S, anticorps MP100, anticorps Psa, anticorps R74825, anticorps goku, anticorps kallikrein related peptidase 3, anticorps kallikrein 3, anticorps kallikrein 1-related peptidase C3, anticorps kallikrein B, plasma 1, anticorps aminopeptidase puromycin sensitive, anticorps KLK3, anticorps Klk1c3, anticorps Klkb1, anticorps Npepps

Sujet: This antibody recognizes a single protein of 33-34  kDa, identified as the Prostate specific antigen. This PSA antibody is highly specific and stains prostatic secretory and ductal epithelium in both normal and neoplastic tissues. Prostate specific antigen is a chymotrypsin-like serine protease (kallikrein family) exclusively produced by the prostate epithelium, and abundant in seminal fluid. PSA can be detected in the sera of patients with prostatic carcinoma. It is predominantly complexed to a liver-derived serine protease inhibitor, alpha-1-antichymotrypsin (ACT). A higher proportion of serum PSA is complexed to ACT in prostate cancer than in benign hyperplasia.

ID gène: 354

Pathways: Système du Complément