Topoisomerase II alpha anticorps
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- Antigène Voir toutes Topoisomerase II alpha (TOP2A) Anticorps
- Topoisomerase II alpha (TOP2A) (Topoisomerase (DNA) II alpha 170kDa (TOP2A))
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Reactivité
- Humain
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Hôte
- Souris
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Clonalité
- Monoclonal
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Conjugué
- Cet anticorp Topoisomerase II alpha est non-conjugé
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Application
- Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS)
- Attributs du produit
- This mAb recognizes a 170 kDa protein which is identified as Topoisomerase IIa, and shows no cross-reaction with Topoisomerase IIb or I. Topo IIa plays important roles in synthesis and transcription of DNA as well as chromosomal segregation during mitosis. It is reported to be a sensitive and specific marker of late S-, G2- & M-phases in transformed and developmentally regulated normal cells. Topo IIa is also implicated in drug resistance of tumor cells.
- Purification
- Protein G affinity chromatography
- Immunogène
- Human partial recombinant protein was used as the immunogen for this TOP2A antibody.
- Clone
- TPM2A-1
- Isotype
- IgG2b kappa
- Top Product
- Discover our top product TOP2A Anticorps primaire
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- Indications d'application
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The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the TOP2A antibody to be titered up or down for optimal performance.
1. Staining of FFPE tissue requires boiling sections in 10 mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.\. FACS: 0.5-1 μg/10^6 cells,IF: 1-2 μg/mL,Western blot: 1-2 μg/mL,IHC (FFPE): 0.5-1 μg/mL (1)
- Restrictions
- For Research Use only
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- Concentration
- 0.2 mg/mL
- Buffer
- 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Stock
- 4 °C,-20 °C
- Stockage commentaire
- Store the TOP2A antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
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- Antigène
- Topoisomerase II alpha (TOP2A) (Topoisomerase (DNA) II alpha 170kDa (TOP2A))
- Autre désignation
- TOP2A (TOP2A Produits)
- Synonymes
- anticorps TOP2, anticorps TP2A, anticorps TOP2A, anticorps Top-2, anticorps TOP-2, anticorps F12B17.110, anticorps F12B17_110, anticorps thimet metalloendopeptidase 2, anticorps fc08c07, anticorps wu:fc08c07, anticorps DNA topoisomerase II alpha, anticorps DNA topoisomerase II, alpha isozyme, anticorps topoisomerase (DNA) II alpha, anticorps Zincin-like metalloproteases family protein, anticorps TOP2A, anticorps Bm1_08705, anticorps Top2a, anticorps AT5G10540, anticorps top2a
- Sujet
- This mAb recognizes a 170 kDa protein which is identified as Topoisomerase IIa, and shows no cross-reaction with Topoisomerase IIb or I. Topo IIa plays important roles in synthesis and transcription of DNA as well as chromosomal segregation during mitosis. It is reported to be a sensitive and specific marker of late S-, G2- & M-phases in transformed and developmentally regulated normal cells. Topo IIa is also implicated in drug resistance of tumor cells.
- ID gène
- 7153
- Pathways
- Cycle Cellulaire, Mitotic G1-G1/S Phases
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