HSPB2 anticorps (Middle Region)

N° du produit ABIN3031147

Détail du produit anti-HSPB2 anticorps

Antigène: HSPB2 (Heat Shock 27kDa Protein 2 (HSPB2))

Épitope: Middle Region

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp HSPB2 est non-conjugé

Application: Western Blotting (WB), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Purification: Antigen affinity

Immunogène: An amino acid sequence from the middle region of human HSPB2 (RHPQRLDRHGFVSREFC) was used as the immunogen for this HSPB2 antibody (100% homologous in human, mouse and rat).

Isotype: IgG

Information d'application

Indications d'application: The stated application concentrations are suggested starting amounts. Titration of the HSPB2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 0.5-1 μg/mL,IHC (Frozen): 0.5-1 μg/mL,Immunocytochemistry: 0.5-1 μg/mL

Restrictions: For Research Use only

Stockage

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Stock: -20 °C

Stockage commentaire: After reconstitution, the HSPB2 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Détails sur HSPB2

Antigène: HSPB2 (Heat Shock 27kDa Protein 2 (HSPB2))

Autre désignation: HSPB2 (HSPB2 Produits)

Synonymes: anticorps zgc:112532, anticorps HSP27, anticorps Hs.78846, anticorps LOH11CR1K, anticorps MKBP, anticorps 27kDa, anticorps 2810021G24Rik, anticorps heat shock protein family B (small) member 2, anticorps heat shock protein, alpha-crystallin-related, b2, anticorps heat shock protein 2, anticorps HSPB2, anticorps hspb2, anticorps Hspb2

Sujet: Alpha-B-crystallin is a member of the alpha-crystallin/small heat-shock protein (HSP) family and under various neuropathologic conditions accumulates in reactive astrocytes and degenerating neurons. In the 5-prime flanking region of the CRYAB gene on 11q22-q23, where a constitutive DNaseI hypersensitive site is located, Iwaki et al.(1997) identified a gene transcribed in the opposite direction. Analysis of its mRNA structure by RT-PCR and 5-prime/3-prime RACE revealed that this gene is composed of 2 exons and encodes a new member of the alpha-crystallin/hsp family. They called it Heat shock 27 protein 2 or Heat shock protein beta 2 and termed the gene HSPB2. Iwaki et al.(1997) also determined the complete genomic structure of the rat homolog of HSPB2. Southern blot analysis demonstrated that HSPB2 is expressed preferentially in skeletal muscle and heart but not in the lens, while the neighboring CRYABgene is highly expressed in all three tissues. The two related genes are arranged in a head-to-head manner with an intergenic sequence of less than 1 kb, raising a possibility of shared regulatory elements for their expression.

UniProt: Q16082